{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T01:06:38Z","timestamp":1768266398115,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,10,25]],"date-time":"2017-10-25T00:00:00Z","timestamp":1508889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish Ministry of Economy and Competitiveness","award":["JR14\/00036"],"award-info":[{"award-number":["JR14\/00036"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Scant information is available about how transplant centers are managing their use of quantitative molecular testing (QNAT<\/jats:styled-content>) assays for active cytomegalovirus (CMV<\/jats:styled-content>) infection monitoring in solid organ transplant (SOT<\/jats:styled-content>) recipients. The current study was aimed at gathering information on current practices in the management of CMV<\/jats:styled-content> infection across European centers in the era of molecular testing assays.<\/jats:p><\/jats:sec>Methods<\/jats:title>A questionnaire\u2010based cross\u2010sectional survey study was conducted by the European Study Group of Infections in Immunocompromised Hosts (ESGICH<\/jats:styled-content>) of the Society of Clinical Microbiology and Infectious Diseases (ESCMID<\/jats:styled-content>). The invitation and a weekly reminder with a personal link to an Internet service provider (https:\/\/es.surveymonkey.com\/<\/jats:ext-link>) was sent to transplant physicians, transplant infectious diseases specialists, and clinical virologists working at 340 European transplant centers.<\/jats:p><\/jats:sec>Results<\/jats:title>Of the 1181 specialists surveyed, a total of 173 responded (14.8%): 73 transplant physicians, 57 transplant infectious diseases specialists, and 43 virologists from 173 institutions located at 23 different countries. The majority of centers used QNAT<\/jats:styled-content> assays for active CMV<\/jats:styled-content> infection monitoring. Most centers preferred commercially available real\u2010time polymerase chain reaction (RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>) assays over laboratory\u2010developed procedures for quantifying CMV DNA<\/jats:styled-content> load in whole blood or plasma. Use of a wide variety of DNA<\/jats:styled-content> extraction platforms and RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content> assays was reported. All programs used antiviral prophylaxis, preemptive therapy, or both, according to current guidelines. However, the centers used different criteria for starting preemptive antiviral treatment, for monitoring systemic CMV DNA<\/jats:styled-content> load, and for requesting genotypic assays to detect emerging CMV<\/jats:styled-content>\u2010resistant variants.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Significant variation in CMV<\/jats:styled-content> infection management in SOT<\/jats:styled-content> recipients still remains across European centers in the era of molecular testing. International multicenter studies are required to achieve commutability of CMV<\/jats:styled-content> testing and antiviral management procedures.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.12773","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T22:10:47Z","timestamp":1504217447000},"source":"Crossref","is-referenced-by-count":38,"title":["Cytomegalovirus infection management in solid organ transplant recipients across European centers in the time of molecular diagnostics: An ESGICH<\/scp> survey"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-4110","authenticated-orcid":false,"given":"David","family":"Navarro","sequence":"first","affiliation":[{"name":"Microbiology Service Hospital Cl\u00ednico Universitario, Institute for Research INCLIVA Valencia Spain"},{"name":"Department of Microbiology School of Medicine University of Valencia Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael","family":"San\u2010Juan","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases Instituto de Investigaci\u00f3n Hospital 12 de Octubre (i+12) University Hospital 12 de Octubre Universidad Complutense Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oriol","family":"Manuel","sequence":"additional","affiliation":[{"name":"Transplantation Center and Service of Infectious Diseases University Hospital of Lausanne CHUV Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estela","family":"Gim\u00e9nez","sequence":"additional","affiliation":[{"name":"Microbiology Service Hospital Cl\u00ednico Universitario, Institute for Research INCLIVA Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0315-8001","authenticated-orcid":false,"given":"Mario","family":"Fern\u00e1ndez\u2010Ruiz","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases Instituto de Investigaci\u00f3n Hospital 12 de Octubre (i+12) University Hospital 12 de Octubre Universidad Complutense Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans H.","family":"Hirsch","sequence":"additional","affiliation":[{"name":"Transplantation & Clinical Virology Department Biomedicine University of Basel Basel Switzerland"},{"name":"Infectious Diseases & Hospital Epidemiology University Hospital Basel Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo Antonio","family":"Grossi","sequence":"additional","affiliation":[{"name":"National Centre for Transplantation Department of Medicine and Surgery, Infectious and Tropical Diseases Unit 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