{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T05:05:30Z","timestamp":1771650330881,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T00:00:00Z","timestamp":1513814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Curetis N.V.\/Curetis GmbH (Germany)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2018,2]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Non\u2010viral lower respiratory tract infections (LRTI<\/jats:styled-content>) are common among lung transplant (LT<\/jats:styled-content>x) recipients with increased mortality. Early pathogen identification is crucial to guide therapy and avoid adverse events. Results from cultures may require up to 72\u00a0hours. Multiplex polymerase chain reaction assay (PCR<\/jats:styled-content>) may allow faster pathogen identification, but its utility in LT<\/jats:styled-content>x recipients with suspected non\u2010viral LRTI<\/jats:styled-content> remains unclear.<\/jats:p><\/jats:sec>Methods<\/jats:title>In a prospective open\u2010label observational trial, LT<\/jats:styled-content>x recipients presenting with suspected LRTI<\/jats:styled-content> received bronchoscopy with bronchoalveolar lavage (BAL<\/jats:styled-content>). Samples were simultaneously analyzed by multiplex PCR<\/jats:styled-content> (Curetis P55 Pneumonia) and cultures for pathogen identification. Time to result notification for PCR<\/jats:styled-content> and cultures and time to final diagnosis were recorded. Definition of non\u2010viral LRTI<\/jats:styled-content> was isolation of a lung\u2010pathogenic non\u2010viral pathogen by either method.<\/jats:p><\/jats:sec>Results<\/jats:title>Forty\u2010eight patients were included, with 32 (67%) having a lung\u2010pathogenic isolate by conventional cultures. In 17\/32 pathogen identification was identical on PCR<\/jats:styled-content>, in 12\/32 (37%) PCR<\/jats:styled-content> returned negative, and in 3\/32 (10%) PCR<\/jats:styled-content> and culture identified different pathogens. Sensitivity and specificity with 95% confidence intervals for pathogen isolation for PCR<\/jats:styled-content> were 66% (47\u201081) and 100% (79\u2010100), respectively. The positive predictive value for PCR<\/jats:styled-content> was 100% (84\u2010100) and the negative predictive value was 59% (39\u201078). PCR<\/jats:styled-content> results were available after 21.2\u00a0hours (interquartile range [IQR<\/jats:styled-content>] 19.3\u201065.7) vs 23\u00a0hours (IQR<\/jats:styled-content> 21.1\u201067.4) from cultures (P<\/jats:italic>\u00a0<\u00a0.0001). The time to final diagnosis of non\u2010viral LRTI<\/jats:styled-content> was 22.9\u00a0hours (20.4\u201037.5).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In LT<\/jats:styled-content>x recipients with suspected non\u2010viral LRTI<\/jats:styled-content>, multiplex PCR<\/jats:styled-content> had a lower sensitivity than cultures. Results were available 2\u00a0hours earlier.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.12811","type":"journal-article","created":{"date-parts":[[2017,11,14]],"date-time":"2017-11-14T16:48:43Z","timestamp":1510678123000},"source":"Crossref","is-referenced-by-count":11,"title":["DNA<\/scp>\u2010based testing in lung transplant recipients with suspected non\u2010viral lower respiratory tract infection: A prospective observational study"],"prefix":"10.1111","volume":"20","author":[{"given":"Nora","family":"Drick","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7373-752X","authenticated-orcid":false,"given":"Benjamin","family":"Seeliger","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Greer","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School Hannover Germany"},{"name":"Biomedical Research in End\u2010Stage and Obstructive Lung Disease (BREATH) German Centre for Lung Research (DZL) Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin A.","family":"Bollmann","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Ziesing","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hospital Hygiene Hannover Medical School Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Welte","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School Hannover Germany"},{"name":"Biomedical Research in End\u2010Stage and Obstructive Lung Disease (BREATH) German Centre for Lung Research (DZL) Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Gottlieb","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Hannover Medical School Hannover Germany"},{"name":"Biomedical Research in End\u2010Stage and Obstructive Lung Disease (BREATH) German Centre for Lung Research (DZL) Hannover 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