{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T15:54:45Z","timestamp":1767974085577,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,5,14]],"date-time":"2018-05-14T00:00:00Z","timestamp":1526256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["FAPESP 2010\/51110\u20102"],"award-info":[{"award-number":["FAPESP 2010\/51110\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (<jats:styled-content style=\"fixed-case\">cPCR<\/jats:styled-content>) and real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>). Polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>) targeting the 18S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene for detection of <jats:italic>Strongyloides stercoralis<\/jats:italic> infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods. <jats:italic>S.\u00a0stercoralis<\/jats:italic> larvae were visualized in 15\/150 (10.0%) transplant candidates by parasitological methods. <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> from <jats:italic>S.\u00a0stercoralis<\/jats:italic> was amplified in 26\/150 (17.3%) and 49\/150 (32.7%) stool samples of transplant candidates, using <jats:styled-content style=\"fixed-case\">cPCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>, respectively. The results suggest that molecular methods, especially <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>, should be used as an additional tool for diagnostic of <jats:italic>S.\u00a0stercoralis<\/jats:italic> infection among transplant candidates.<\/jats:p>","DOI":"10.1111\/tid.12909","type":"journal-article","created":{"date-parts":[[2018,4,21]],"date-time":"2018-04-21T03:44:20Z","timestamp":1524282260000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Molecular diagnosis of <i>Strongyloides stercoralis<\/i> among transplant candidates"],"prefix":"10.1111","volume":"20","author":[{"given":"Fabiana M.","family":"Paula","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica (LIM\/06 \u2013 Laborat\u00f3rio de Imunopatologia da Esquistossomose) Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"},{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda M.","family":"Malta","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo USP  S\u00e3o Paulo Brazil"},{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica (LIM\/07 \u2013 Laborat\u00f3rio de Gastroenterologia e Hepatologia Tropical) Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscilla D.","family":"Marques","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica (LIM\/06 \u2013 Laborat\u00f3rio de Imunopatologia da Esquistossomose) Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"},{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gessica B.","family":"Melo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica (LIM\/06 \u2013 Laborat\u00f3rio de Imunopatologia da Esquistossomose) Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"},{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo A.","family":"Corral","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica (LIM\/06 \u2013 Laborat\u00f3rio de Imunopatologia da Esquistossomose) Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maiara","family":"Gottardi","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica (LIM\/06 \u2013 Laborat\u00f3rio de Imunopatologia da Esquistossomose) Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o R. R.","family":"Pinho","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo USP  S\u00e3o Paulo Brazil"},{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica (LIM\/07 \u2013 Laborat\u00f3rio de Gastroenterologia e Hepatologia Tropical) Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elenice M. N.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia da Divis\u00e3o de Laborat\u00f3rio Central Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera L. P.","family":"Castilho","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Parasitologia da Divis\u00e3o de Laborat\u00f3rio Central Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ligia C.","family":"Pierrotti","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edson","family":"Abdala","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia F.","family":"Costa","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro P.","family":"Chieffi","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias M\u00e9dicas, Santa Casa  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2227-3037","authenticated-orcid":false,"given":"Ronaldo C. B.","family":"Gryschek","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00e3o M\u00e9dica (LIM\/06 \u2013 Laborat\u00f3rio de Imunopatologia da Esquistossomose) Hospital das Cl\u00ednicas da Faculdade de Medicina USP  S\u00e3o Paulo Brazil"},{"name":"Instituto de Medicina Tropical de S\u00e3o Paulo USP  S\u00e3o Paulo Brazil"},{"name":"Faculdade de Medicina, USP  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2009.02.013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003018"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268814001563"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.208-217.2004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e3283551dbd"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.967"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2008.12.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182013002035"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2014.10.020"},{"key":"e_1_2_7_11_1","volume-title":"Diagnostic Medical Parasitology","author":"Garcia LS","year":"2001"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1991.45.518"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2999980"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370214523880"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.07.019"},{"key":"e_1_2_7_16_1","first-page":"23","article-title":"Molecular diagnosis of Strongyloides stercoralis infection by PCR detection of specific DNA in human stool samples","volume":"6","author":"Moghaddassani H","year":"2011","journal-title":"Iran J Parasitol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.12.012"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2010.11.005"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06012-11"}],"container-title":["Transplant Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftid.12909","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.12909","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T15:54:52Z","timestamp":1694966092000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tid.12909"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,14]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1111\/tid.12909"],"URL":"https:\/\/doi.org\/10.1111\/tid.12909","archive":["Portico"],"relation":{},"ISSN":["1398-2273","1399-3062"],"issn-type":[{"value":"1398-2273","type":"print"},{"value":"1399-3062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,14]]},"assertion":[{"value":"2017-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12909"}}