{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:27:47Z","timestamp":1790360867827,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,9]],"date-time":"2018-11-09T00:00:00Z","timestamp":1541721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671574"],"award-info":[{"award-number":["81671574"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81501381"],"award-info":[{"award-number":["81501381"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470975"],"award-info":[{"award-number":["81470975"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To explore the role of regulatory T (T<jats:sub>reg<\/jats:sub>) cells in the establishment of immune tolerance induced by donor\u2010specific transfusion (DST) in mice with skin\u2010heart transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C57BL\/6 mice received DST of splenocytes from CD47<jats:sup>+\/+<\/jats:sup> or CD47<jats:sup>\u2212\/\u2212<\/jats:sup> H\u20102<jats:sup>bm1<\/jats:sup> mice or no DST 7\u00a0days before skin\u2010heart transplantation from major histocompatibility complex class I\u2010mismatched H\u20102<jats:sup>bm1<\/jats:sup> donors. The number and proportion of T<jats:sub>reg<\/jats:sub> cells in graft and lymphoid organs were measured by flow cytometry (FACS) and immunohistochemistry (IHC). The inhibitory function of T<jats:sub>reg<\/jats:sub> cells and anti\u2010donor T\u2010cell responses were assessed by mixed lymphocyte reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed that mean survival time (MST) of skin or heart graft was significantly longer in C57BL\/6 mice which received DST from CD47<jats:sup>+\/+<\/jats:sup> H\u20102<jats:sup>bm1<\/jats:sup> mice than from CD47<jats:sup>\u2212\/\u2212<\/jats:sup> H\u20102<jats:sup>bm1<\/jats:sup> mice. By FACS, we found that the number of T<jats:sub>reg<\/jats:sub> cells in spleen was increased significantly in mice which received CD47<jats:sup>\u2212\/\u2212<\/jats:sup> DST compared to mice which received CD47<jats:sup>+\/+<\/jats:sup> DST. However, the percentages of T<jats:sub>reg<\/jats:sub> cells in total splenocytes and lymph node cells were significantly higher in mice that received CD47<jats:sup>+\/+<\/jats:sup> DST than mice which received CD47<jats:sup>\u2212\/\u2212<\/jats:sup> DST. Immunohistochemistry showed an increased heart grafts infiltration of T<jats:sub>reg<\/jats:sub> cells in the recipients with CD47<jats:sup>\u2212\/\u2212<\/jats:sup> DST, but not CD47<jats:sup>+\/+<\/jats:sup> DST. Supporting this, we found that donor T\u2010cell proliferation was significantly suppressed in mice which received CD47<jats:sup>+\/+<\/jats:sup> DST compared to mice which received CD47<jats:sup>\u2212\/\u2212<\/jats:sup> DST. There was no difference of inhibitory function of T<jats:sub>reg<\/jats:sub> cells between these two groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results indicated that CD47 expression on DST cells plays an important role in the induction of immune tolerance in mice with skin\u2010heart transplantation. Increased percentage of T<jats:sub>reg<\/jats:sub> cells may contribute to immune tolerance induced by CD47<jats:sup>+\/+<\/jats:sup> DST.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.13012","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T13:26:31Z","timestamp":1539609991000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Role of regulatory T cells in CD47\/donor\u2010specific transfusion\u2010induced immune tolerance in skin\u2010heart transplantation mice"],"prefix":"10.1111","volume":"21","author":[{"given":"Yu","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Pathology, China\u2010Japan Union Hospital Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Urology The First Hospital of Jilin University Changchun 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