{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:54:22Z","timestamp":1791561262420,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Solid organ transplant recipients are highly susceptible to <jats:italic>Toxoplasma gondii<\/jats:italic> infection. We aimed to describe the 12\u2010month follow\u2010up risk of seroconversion in renal transplant recipients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Anti\u2010<jats:italic>T\u00a0gondii<\/jats:italic> antibodies were investigated in donors and recipients of renal transplants. In donors, anti\u2010<jats:italic>T\u00a0gondii<\/jats:italic> were evaluated before transplantation. In recipients, anti\u2010<jats:italic>T\u00a0gondii<\/jats:italic> were monitored over a 12\u2010month period to evaluate potential seroconversion or reactivation. IgG and IgM anti\u2010<jats:italic>T gondii<\/jats:italic> antibodies were investigated through enzyme immunoassay and Western blot. Molecular diagnosis was performed on peripheral blood leukocytes using PCR to amplify fragments corresponding to the <jats:italic>T\u00a0gondii<\/jats:italic> B1 gene and the repetitive 529\u2010bp element.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The basal frequency of seropositive IgG anti\u2010<jats:italic>T\u00a0gondii<\/jats:italic> antibodies was higher in donors than in recipients (38.4% vs 25.2%; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.03). During the 12\u2010month follow\u2010up, the accumulated seroconversion to IgG and IgM antibodies was 3\/99 (3.0%), and the accumulated reactivation was 11\/99 (11.0%). None of the samples exhibited positivity to <jats:italic>T\u00a0gondii<\/jats:italic> DNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study showed that there is an increased risk of seroconversion or reactivation in renal transplant recipients over a 12\u2010month follow\u2010up. Our data suggest that prophylaxis with trimethoprim and sulfamethoxazole effectively prevented toxoplasmosis, since neither <jats:italic>T\u00a0gondii<\/jats:italic> DNA nor clinical toxoplasmosis was detected.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.13139","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T18:49:27Z","timestamp":1562266167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Toxoplasma<\/i> infection in kidney donors and transplant recipients from Western Mexico: A one\u2010year follow\u2010up"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5581-4322","authenticated-orcid":false,"given":"Mar\u00eda Luz","family":"Galv\u00e1n\u2010Ram\u00edrez","sequence":"first","affiliation":[{"name":"Department of Physiology, Neurophysiology Laboratory Health Sciences University Center, University of Guadalajara Guadalajara M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura V.","family":"S\u00e1nchez\u2010Orozco","sequence":"additional","affiliation":[{"name":"Institute for Molecular Biology in Medicine Health Sciences University Center, University of Guadalajara Guadalajara M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Andrade\u2010Sierra","sequence":"additional","affiliation":[{"name":"Department of Nephrology, and Transplant Western National Medical Center of the Mexican Social Security Institute Guadalajara Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador","family":"Mendoza\u2010Cabrera","sequence":"additional","affiliation":[{"name":"Department of Nephrology, and Transplant Western National Medical Center of the Mexican Social Security Institute Guadalajara Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Alberto","family":"Evangelista\u2010Carrillo","sequence":"additional","affiliation":[{"name":"Department of Nephrology, and Transplant Western National Medical Center of the Mexican Social Security Institute Guadalajara Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura R.","family":"Rodr\u00edguez P\u00e9rez","sequence":"additional","affiliation":[{"name":"Department of Physiology, Neurophysiology Laboratory Health Sciences University Center, University of Guadalajara Guadalajara M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"Chiquete","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Psychiatry Salvador Zubiran National Institute of Medical Sciences and Nutrition Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Armend\u00e1riz\u2010Borunda","sequence":"additional","affiliation":[{"name":"Institute for Molecular Biology in Medicine Health Sciences University Center, University of Guadalajara Guadalajara M\u00e9xico"},{"name":"Tecnologico de Monterrey, Campus Guadalajara Guadalajara Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,25]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"Galv\u00e1n\u2010Ram\u00edrezML Mondrag\u00f3n\u2010FloresR.Toxoplasmosis humana. 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