{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T06:38:34Z","timestamp":1768804714186,"version":"3.49.0"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T00:00:00Z","timestamp":1571875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["K23 AI 124464"],"award-info":[{"award-number":["K23 AI 124464"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R37 AI 051652"],"award-info":[{"award-number":["R37 AI 051652"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In kidney transplant recipients, gastrointestinal (GI) pathogens in feces are only evaluated during diarrheal episodes. Little is known about the prevalence of GI pathogens in asymptomatic individuals in this population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We recruited 142 kidney transplant recipients who provided a non\u2010diarrheal fecal sample within the first 10\u00a0days after transplantation. The specimens were evaluated for GI pathogens using the BioFire<jats:sup>\u00ae<\/jats:sup> FilmArray<jats:sup>\u00ae<\/jats:sup> GI Panel (BioFire Diagnostics, LLC), which tests for 22 pathogens. The fecal microbiome was also characterized using 16S rRNA gene sequencing of the V4\u2010V5 hypervariable region. We evaluated whether detection of <jats:italic>Clostridioides difficile<\/jats:italic> and other GI pathogens was associated with post\u2010transplant diarrhea within the first 3\u00a0months after transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 142 subjects, a potential pathogen was detected in 43 (30%) using the GI Panel. The most common organisms detected were <jats:italic>C\u00a0difficile<\/jats:italic> (n\u00a0=\u00a024, 17%), enteropathogenic <jats:italic>Escherichia coli<\/jats:italic> (n\u00a0=\u00a08, 6%), and norovirus (n\u00a0=\u00a05, 4%). Detection of a pathogen on the GI panel or detection of <jats:italic>C\u00a0difficile<\/jats:italic> alone was not associated with future post\u2010transplant diarrhea (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a0.05). The estimated number of gut bacterial species was significantly lower in subjects colonized with <jats:italic>C\u00a0difficile<\/jats:italic> than those not colonized with\u00a0a GI pathogen (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Colonization with GI pathogens, particularly <jats:italic>C\u00a0difficile<\/jats:italic>, is common at the time of kidney transplantation but does not predict subsequent diarrhea. Detection of <jats:italic>C\u00a0difficile<\/jats:italic> carriage was associated with decreased microbial diversity and may be a biomarker of gut dysbiosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.13167","type":"journal-article","created":{"date-parts":[[2019,9,10]],"date-time":"2019-09-10T10:55:00Z","timestamp":1568112900000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Gastrointestinal pathogen colonization and the microbiome in asymptomatic kidney transplant recipients"],"prefix":"10.1111","volume":"21","author":[{"given":"Lars F.","family":"Westblade","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Weill Cornell Medicine New York NY USA"},{"name":"Division of Infectious Diseases Department of Medicine Weill Cornell Medicine New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4711-5334","authenticated-orcid":false,"given":"Michael J.","family":"Satlin","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine Weill Cornell Medicine New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shady","family":"Albakry","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brittany","family":"Botticelli","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Robertson","sequence":"additional","affiliation":[{"name":"NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tricia","family":"Alston","sequence":"additional","affiliation":[{"name":"NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Magruder","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa T.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Edusei","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Chan","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Lubetzky","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"},{"name":"Department of Transplantation Medicine Weill Cornell Medical Center NewYork\u2010Presbyterian Hospital New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darshana M.","family":"Dadhania","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"},{"name":"Department of Transplantation Medicine Weill Cornell Medical Center NewYork\u2010Presbyterian Hospital New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric G.","family":"Pamer","sequence":"additional","affiliation":[{"name":"Infectious Disease Service Department of Medicine Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manikkam","family":"Suthanthiran","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"},{"name":"Department of Transplantation Medicine Weill Cornell Medical Center NewYork\u2010Presbyterian Hospital New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9612-5742","authenticated-orcid":false,"given":"John R.","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension NewYork Presbyterian Hospital \u2010 Weill Cornell Medical Center New York NY USA"},{"name":"Department of Transplantation Medicine Weill Cornell Medical Center NewYork\u2010Presbyterian Hospital New York NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199908150-00011"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2007.00237.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu880"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124483"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1526924816655073"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12918"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14974"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02674-14"},{"key":"e_1_2_9_10_1","unstructured":"World Health Organization.The treatment of diarrhoea: a manual for physicians and other senior health workers.2005."},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000000370"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2604"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv401"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1274"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamainternmed.2015.4114"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1216064"}],"container-title":["Transplant Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftid.13167","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.13167","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tid.13167","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.13167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T22:24:41Z","timestamp":1694211881000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tid.13167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,24]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["10.1111\/tid.13167"],"URL":"https:\/\/doi.org\/10.1111\/tid.13167","archive":["Portico"],"relation":{},"ISSN":["1398-2273","1399-3062"],"issn-type":[{"value":"1398-2273","type":"print"},{"value":"1399-3062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,24]]},"assertion":[{"value":"2019-05-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13167"}}