{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T02:35:11Z","timestamp":1788489311213,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T00:00:00Z","timestamp":1647388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Mycoplasma hominis<\/jats:italic> can cause significant infections after solid organ transplantation (SOT). Treatment should be guided by susceptibility testing, but conventional lab methods are laborious with prolonged turnaround time (TAT). This case series compares the phenotypic and genotypic susceptibility profiles of <jats:italic>M. hominis<\/jats:italic> isolates identified from SOT patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This is a single\u2010center retrospective study evaluating SOT recipients with confirmed <jats:italic>M. hominis<\/jats:italic> infections. Patients\u2019 demographic, clinical, microbiological, and radiographic data were collected. Culture of <jats:italic>M. hominis<\/jats:italic> isolates was performed according to current Clinical and Laboratory Standards Institute guidelines. Phenotypic susceptibility testing was performed by University of Alabama Diagnostic Mycoplasma Laboratory. Whole genome sequencing (WGS) was performed followed by bioinformatic analysis of known genetic determinants of resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seven SOT recipients with <jats:italic>M. hominis<\/jats:italic> infections were identified. Two out of seven (28.5%) patients had resistance detected by phenotypic susceptibility testing (Case 5 to levofloxacin and Case 7 to tetracycline). Genomic analyses confirmed the presence of mutations in the <jats:italic>parC<\/jats:italic> and <jats:italic>parE<\/jats:italic> topoisomerase genes at positions conferring to fluoroquinolone resistance in the isolate from Case 5, while the tetracycline\u2010resistant isolate from Case 7 harbored the <jats:italic>tetM<\/jats:italic> gene. The median TAT from the date of specimen collection was 24 days for phenotypic susceptibility testing and 14 days for genotypic susceptibility testing. All seven patients received antimicrobials directed toward <jats:italic>M. hominis<\/jats:italic> and recovered with complete resolution of infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>WGS may offer a novel and more rapid methodology for <jats:italic>M. hominis<\/jats:italic> susceptibility testing to help optimize antimicrobial usage, but more data are needed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.13822","type":"journal-article","created":{"date-parts":[[2022,3,7]],"date-time":"2022-03-07T11:36:19Z","timestamp":1646652979000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>Mycoplasma hominis<\/i> infections in solid organ transplant recipients: Clinical characteristics, treatment outcomes, and comparison of phenotypic and genotypic susceptibility profiles"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9783-3567","authenticated-orcid":false,"given":"Sandy Y.","family":"Chang","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine David Geffen School of Medicine at UCLA  Los Angeles California USA"},{"name":"Division of Infectious Diseases Department of Medicine Loma Linda University  Loma Linda California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis K.","family":"Price","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine David Geffen School of Medicine at UCLA  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer E.","family":"Beaird","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Medicine David Geffen School of Medicine at UCLA  Los Angeles California 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