{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:00:21Z","timestamp":1792699221105,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Among kidney transplant recipients (KTR) with BK virus associated nephropathy (BKVN), BKV genotypes\u2019 evolution and anti\u2010BKV humoral response are not well established. We aim to analyze BKV replication and genetic evolution following transplantation, and characterize concomitant anti\u2010BKV\u2010VP1 humoral response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively analyzed 32 cases of biopsy\u2010proven BKVN. Stored plasma and kidney biopsies were tested for BKV viral load, and VP1 sequencing performed on positive samples. BKV\u2013VP1 genotype\u2010specific neutralizing antibodies (NAbs) titers were determined at transplantation and BKVN.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At the time of BKVN diagnosis, BKV viral load was 8.2 log<jats:sub>10<\/jats:sub>IU\/10<jats:sup>6<\/jats:sup> cells and 5.4 log<jats:sub>10<\/jats:sub>IU\/mL in kidney and plasma, respectively. VP1 sequencing identified the same BKV\u2010subtype in both compartments in 31\/32 cases. At the time of transplantation, 8\/20 (40%) of biopsies tested positive for BKV detection, whereas concomitant BKV viremia was negative. VP1 sequencing identified a different subtype compared to BKVN in 5\/6 of these samples. This was confirmed following transplantation: 8 patients had a BKV+ biopsy before BKV viremia, and VP1 sequencing identified a different subtype compared to BKVN in all of them. After the onset of BKV viremia and prior to BKVN diagnosis, the BKV subtype in BKV+ plasma and kidney biopsy was the same as the one isolated at BKVN. BKV\u2013VP1 NAbs titers were significantly higher at the time of BKVN compared to transplantation (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.0031), with similar titers across genotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Altogether, our data suggest that among some KTR with BKVN, the BKV genotype from the donor may not be responsible for BKVN pathogenesis.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tid14012-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.14012","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T11:14:45Z","timestamp":1675768485000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["BK virus genotypes and humoral response in kidney transplant recipients with BKV associated nephropathy"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7247-3358","authenticated-orcid":false,"given":"Julien","family":"Gras","sequence":"first","affiliation":[{"name":"Infectious Disease Department APHP\u2010Saint\u2010Louis Hospital  Paris France"},{"name":"INSERM U944, Biology of Emerging Viruses Team Institut de Recherche Saint Louis, APHP\u2010Saint\u2010Louis Hospital  Paris France"},{"name":"Universit\u00e9 Paris Cit\u00e9  Paris 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Department APHP\u2010Saint Louis Hospital  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5766-046X","authenticated-orcid":false,"given":"Jean Luc","family":"Taupin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9  Paris France"},{"name":"Immunology Department APHP\u2010Saint Louis Hospital  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Desgrandchamps","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9  Paris France"},{"name":"Urology Department APHP\u2010Saint Louis Hospital  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Verine","sequence":"additional","affiliation":[{"name":"Pathology Department APHP\u2010Saint Louis Hospital  Paris 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