{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T05:05:39Z","timestamp":1771650339017,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T00:00:00Z","timestamp":1688947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016739","name":"BioFire Diagnostics","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Culture of bronchoalveolar lavage (BAL) specimens takes time to report. We tested whether a molecular diagnostic test could accelerate donor lung assessment and treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared BioFire\u00a0Film Array\u00a0Pneumonia Panel (BFPP) with <jats:italic>standard of care<\/jats:italic> (SOC) tests on lung allograft samples at three time points: (1) donor BAL at organ recovery, (2) donor bronchial tissue and airway swab at implantation, and (3) first recipient BAL following lung implantation. Primary outcomes were the difference in time to result (Wilcoxon signed\u2010ranked tests) and the agreement in results between BFPP and SOC assays (Gwet's agreement coefficient).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We enrolled 50 subjects. In donor lung BAL specimens, BFPP detected 52 infections (14 out of 26 pathogens in the panel). Viral and bacterial BFPP results were reported 2.4 h (interquartile range, IQR 2.0\u20136.4) following BAL versus 4.6 h (IQR 1.9\u20136.0, <jats:italic>p<\/jats:italic> = 0.625) for OPO BAL <jats:italic>viral<\/jats:italic> SOC results and 66 h (IQR 47\u201387, <jats:italic>p<\/jats:italic> &lt; .0001) for OPO BAL <jats:italic>bacterial<\/jats:italic> SOC results. Although there was high overall agreement of results between BAL\u2013BFPP versus OPO BAL\u2013SOC tests (Gwet's AC <jats:italic>p<\/jats:italic> &lt; .001 for all), the level of agreement differed among 26 pathogens designed in BFPP and differed by types of specimens. BFPP could not detect many infections identified by SOC assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>BFPP decreased time to detection of lung pathogens among donated lungs, but it cannot replace SOC tests due to the limited number of pathogens in the panel.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tid14091-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.14091","type":"journal-article","created":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T18:31:37Z","timestamp":1689013897000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Biofire pneumonia panel in lung donors: faster detection but limited pathogens"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2608-0152","authenticated-orcid":false,"given":"Anh","family":"Nguyen","sequence":"first","affiliation":[{"name":"Department of Surgery School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joy","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Surgery School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin","family":"Isaza","sequence":"additional","affiliation":[{"name":"Department of Surgery School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niel","family":"Panchal","sequence":"additional","affiliation":[{"name":"Department of Surgery School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fred","family":"Deiter","sequence":"additional","affiliation":[{"name":"Department of Medicine School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Hoover","sequence":"additional","affiliation":[{"name":"Department of Medicine School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binh","family":"Trinh","sequence":"additional","affiliation":[{"name":"Department of Surgery School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve R.","family":"Hays","sequence":"additional","affiliation":[{"name":"Department of Medicine School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey A.","family":"Golden","sequence":"additional","affiliation":[{"name":"Department of Medicine School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7789-5047","authenticated-orcid":false,"given":"Jonathan P.","family":"Singer","sequence":"additional","affiliation":[{"name":"Department of Medicine School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Brzezinski","sequence":"additional","affiliation":[{"name":"Department of Anesthesia and Perioperative Care School of Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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