{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T23:13:36Z","timestamp":1777331616399,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Donor\u2010derived dengue infections present significant challenges to organ transplantation, particularly in endemic regions like Singapore. Although primarily transmitted by Aedes mosquitoes, dengue can also be transmitted through organ transplantation, occasionally with fatal outcomes. This study aims to evaluate the outcomes and evolution of dengue screening protocols for potential deceased donors in Singapore from 2006 to 2022.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Initially, screening was done via dengue immunoglobulin M (IgM), targeting donors with specific clinical criteria (thrombocytopenia, drop in platelet count, prolonged prothrombin time\/partial thromboplastin time, and discretion of the transplant team), later transitioning to blood dengue reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) in 2007 with similar criteria, and subsequently universal screening in 2016. In 2021, urine dengue RT\u2010PCR was added following a case of donor\u2010derived dengue infection from an aviremic but viruric donor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of 431 potential deceased donors, 395 (91.6%) underwent dengue screening, with six (1.5%) testing positive for dengue. In 2006, three positive screens were identified: two through dengue IgM and one via blood dengue RT\u2010PCR; subsequent years saw one positive screen each in 2007, 2008, and 2019 via blood dengue RT\u2010PCR. Potential deceased donors with a positive blood dengue screen were rejected as solid organ and tissue donors. Those with negative blood dengue RT\u2010PCR but positive urine dengue RT\u2010PCR would be rejected as kidney donors, but the use of other organs and tissues was at the discretion of the transplantation team.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The optimal screening protocol remains uncertain, but our findings suggest that a universal screening strategy utilizing both blood and urine dengue RT\u2010PCR could be considered in dengue\u2010endemic countries.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tid14356-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/tid.14356","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T11:33:59Z","timestamp":1723808039000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Donor\u2010derived dengue infections \u2013 A review of screening protocol and outcomes in an endemic country"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7167-3216","authenticated-orcid":false,"given":"Sophie Seine Xuan","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Singapore General Hospital  Singapore Singapore"},{"name":"Duke\u2010NUS Medical School  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samsudin Bin","family":"Nordin","sequence":"additional","affiliation":[{"name":"National Organ Transplant Unit Ministry of Health  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chee\u2010Kiat","family":"Tan","sequence":"additional","affiliation":[{"name":"Duke\u2010NUS Medical School  Singapore Singapore"},{"name":"National Organ Transplant Unit Ministry of Health  Singapore Singapore"},{"name":"Department of Gastroenterology and Hepatology Singapore General Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thuan Tong","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Singapore General Hospital  Singapore Singapore"},{"name":"Duke\u2010NUS Medical School  Singapore Singapore"},{"name":"Singhealth Duke Transplant Centre  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5174-7361","authenticated-orcid":false,"given":"Shimin Jasmine","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Singapore General Hospital  Singapore Singapore"},{"name":"Duke\u2010NUS Medical School  Singapore Singapore"},{"name":"Singhealth Duke Transplant Centre  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kian Sing","family":"Chan","sequence":"additional","affiliation":[{"name":"Duke\u2010NUS Medical School  Singapore Singapore"},{"name":"Department of Microbiology Singapore General Hospital  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1458-9456","authenticated-orcid":false,"given":"Ban Hock","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Singapore General Hospital  Singapore Singapore"},{"name":"Duke\u2010NUS Medical School  Singapore Singapore"},{"name":"Singhealth Duke Transplant Centre  Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2018.01.001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfh601"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15270"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40794\u2010016\u20100032\u2010y"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.16540"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.06557\u201011"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010015\u20102359\u20100"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17\u20100819"},{"key":"e_1_2_11_10_1","unstructured":"National Environment Agency.NEA launches national dengue prevention campaign early to urge continued vigilance and avert a dengue outbreak in 2023 updated March 25 2023. 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