{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T11:38:57Z","timestamp":1793619537080,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2026,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Kidney transplant (KT) recipients at intermediate risk for cytomegalovirus (CMV) infection constitute a potential target for individualized prevention strategies informed by the CMV\u2010specific cell\u2010mediated immunity (CMV\u2010CMI). The optimal method for the functional assessment of CMV\u2010CMI in this group remains unclear.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We included 74 CMV\u2010seropositive KT recipients that did not receive T\u2010cell\u2010depleting induction and were managed by preemptive therapy. CMV\u2010CMI was monitored at baseline and months 1, 3, 6, and 12 by intracellular cytokine staining (ICS) and a interferon (IFN)\u2010\u03b3\u2010release assay (QuantiFERON\u2010CMV [QTF\u2010CMV]). Both methods were compared for discriminative capacity (areas under the receiving operating characteristic curve [auROCs]) and diagnostic accuracy to predict protection against high\u2010level (\u22651000\u00a0IU\/mL) CMV DNAemia and\/or disease.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Eighteen patients (24.3%) experienced high\u2010level CMV DNAemia or disease. There were no significant differences in the discriminative capacity to predict protection of CMV\u2010specific CD8+ (auROC: 0.719) and CD4+ T\u2010cell counts (auROC: 0.664) enumerated by ICS and IFN\u2010\u03b3 production measured by QTF\u2010CMV (auROC: 0.666). Optimal cutoff values of \u22659.8 CMV\u2010specific CD4+ T\u2010cells\/\u00b5L and \u22655.7 CD8+ T\u2010cells\/\u00b5L by ICS yielded excellent specificity (95.7% and 86.9%, respectively) and positive predictive values (PPVs) (>98.0%), but a sensitivity below 60%. A reactive QTF\u2010CMV (IFN\u2010\u03b3 \u22650.2\u00a0IU\/mL) provided good sensitivity (81.6%) and PPV (92.5%), at the expense of a poor specificity (22.2%).<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The discriminative capacity to predict immune protection against clinically relevant CMV infection among intermediate\u2010risk KT recipients was comparable for ICS and QTF\u2010CMV. A selected ICS threshold may provide better specificity than the interpretative cut\u2010off values currently recommended for QTF\u2010CMV.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/tid.14437","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T08:30:17Z","timestamp":1736497817000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Diagnostic Performance of Two Different Techniques to Quantify CMV\u2010Specific Cell\u2010Mediated Immunity in Intermediate\u2010Risk Seropositive Kidney Transplant Recipients"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0315-8001","authenticated-orcid":false,"given":"Mario","family":"Fern\u00e1ndez\u2010Ruiz","sequence":"first","affiliation":[{"name":"Unit of Infectious Diseases Hospital Universitario \u201c12 de Octubre\u201d Instituto de 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Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Medicine School of Medicine Universidad Complutense Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9028-055X","authenticated-orcid":false,"given":"Est\u00e9fani","family":"Garc\u00eda\u2010R\u00edos","sequence":"additional","affiliation":[{"name":"Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA) Consejo Superior de Investigaciones Cient\u00edficas (CSIC) Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8261-6057","authenticated-orcid":false,"given":"Tamara","family":"Ruiz\u2010Merlo","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9356-8102","authenticated-orcid":false,"given":"Natalia","family":"Redondo","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid 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de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Medicine School of Medicine Universidad Complutense Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3446-1991","authenticated-orcid":false,"given":"Rafael","family":"San Juan","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Medicine School of Medicine Universidad Complutense Madrid 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Mar\u00eda","family":"Aguado","sequence":"additional","affiliation":[{"name":"Unit of Infectious Diseases Hospital Universitario \u201c12 de Octubre\u201d Instituto de Investigaci\u00f3n Sanitaria Hospital \u201c12 de Octubre\u201d (imas12) Madrid Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC) Instituto de Salud Carlos III (ISCIII) Madrid Spain"},{"name":"Department of Medicine School of Medicine Universidad Complutense Madrid 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