{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:34:04Z","timestamp":1787506444684,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2025,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n Twenty percent of lung donors in our transplant program carry respiratory carbapenem\u2010resistant (CR) gram\u2010negative bacteria (GNB), most commonly CR\n Acinetobacter baumannii<\/jats:italic>\n . Universal multiplex panel testing of lung transplant donors was introduced in June 2022 as a strategy to expedite CR\u2010GNB detection and optimize perioperative antibiotic prophylaxis. We herein describe our experiences with this approach.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Retrospective single\u2010center cohort study including 53 adult patients who underwent lung\u2010only transplantation between June 2022 and December 2023.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The most common bacteria identified by the multiplex panel were\n Staphylococcus aureus<\/jats:italic>\n (\n n<\/jats:italic>\n \u00a0=\u00a020),\n A. baumannii<\/jats:italic>\n (\n n<\/jats:italic>\n \u00a0=\u00a013),\n Klebsiella pneumoniae<\/jats:italic>\n (\n n<\/jats:italic>\n \u00a0=\u00a013), and\n Pseudomonas aeruginosa<\/jats:italic>\n (\n n<\/jats:italic>\n \u00a0=\u00a010). The panel detected 6\/9\n A. baumannii<\/jats:italic>\n , 2\/2 CR\n K. pneumoniae<\/jats:italic>\n , 1\/1 CR\n P. aeruginosa<\/jats:italic>\n , and 7\/8 methicillin\u2010resistant\n S. aureus<\/jats:italic>\n that were grown on conventional cultures, corresponding to negative predictive values of 94%, 100%, 100%, and 98%. Based on panel or culture results, IV tigecycline was administered as prophylaxis in 19% of patients, colistin in 17%, and novel beta\u2010lactams in 15%.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The multiplex panel rapidly detected donor CR\u2010GNB with a high negative predictive value and resulted in clinical effects of reducing broad\u2010spectrum antibiotic prescriptions and maintaining adequate posttransplant outcomes. Prospective studies with predefined outcomes are needed to compare panel\u2010directed therapy against current standards of care.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/tid.14446","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T09:09:46Z","timestamp":1739783386000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Universal Multiplex Panel Testing of Donor Lungs as a Strategy to Optimize Antibiotic Prophylaxis Against Multidrug\u2010Resistant Bacteria"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0805-5714","authenticated-orcid":false,"given":"Rayid","family":"Abdulqawi","sequence":"first","affiliation":[{"name":"Lung Health Centre Department Organ Transplant Centre of Excellence King Faisal Specialist Hospital and Research Centre Riyadh Saudi 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Al","family":"Duhailib","sequence":"additional","affiliation":[{"name":"College of Medicine Alfaisal University Riyadh Saudi Arabia"},{"name":"Critical Care Medicine Department King Faisal Specialist Hospital and Research Centre Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Althawadi","sequence":"additional","affiliation":[{"name":"Pathology & Laboratory Medicine Department King Faisal Specialist Hospital and Research Centre Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najib M.","family":"Rahman","sequence":"additional","affiliation":[{"name":"Oxford Centre for Respiratory Medicine NIHR Oxford Biomedical Research Centre Oxford Respiratory Trials Unit University of Oxford Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleed","family":"Saleh","sequence":"additional","affiliation":[{"name":"Lung Health Centre Department Organ Transplant Centre of Excellence King 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