{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T07:23:42Z","timestamp":1781076222655,"version":"3.54.1"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T00:00:00Z","timestamp":1753142400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gastrointestinal (GI) cytomegalovirus (CMV) infection is an important cause of morbidity after solid organ transplantation (SOT), and diagnosis mainly relies on histopathology of GI tissue biopsies. CMV detection by quantitative polymerase chain reaction (qPCR) on tissue biopsy is not routinely performed, but potentially holds many practical advantages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We compared the performance of CMV qPCR on fresh GI biopsies to histopathologic identification for the detection of CMV GI disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Sixty\u2010one SOT patients with GI symptoms underwent endoscopic assessment, with tissue biopsies obtained. Eleven patients had proven CMV disease by histopathologic detection. Among them, all had a positive qPCR on tissue biopsy (median of 8.7\u00a0\u00d7\u00a010\n                      <jats:sup>7<\/jats:sup>\n                      IU\/mL [interquartile range {IQR} 3.1\u00a0\u00d7\u00a010\n                      <jats:sup>7<\/jats:sup>\n                      , 18.2\u00a0\u00d7\u00a010\n                      <jats:sup>7<\/jats:sup>\n                      ]). Of the 49 patients with negative histopathology, 27 (55%) had CMV qPCR\u2010positive tissue biopsy specimens (median of 43 604 IU\/mL [IQR 2923, 497 570]). Receiver operating characteristic analysis for optimal threshold value for CMV qPCR on tissue biopsy for diagnosis of proven CMV GI disease was 147 906 IU\/mL (sensitivity 100%, specificity 80%, area under the curve = 0.975).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Compared to histopathologic detection, CMV qPCR on GI tissue biopsy is highly sensitive for the diagnosis of CMV GI disease in SOT patients, making it a potentially useful adjunctive diagnostic tool for rapid diagnosis in this population.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tid.70082","type":"journal-article","created":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T12:50:48Z","timestamp":1753188648000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Utility of Cytomegalovirus Quantitative Polymerase Chain Reaction in Tissue Biopsy for the Diagnosis of Cytomegalovirus Gastrointestinal Disease Among Solid Organ Transplant Recipients"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4508-3827","authenticated-orcid":false,"given":"Lucila","family":"Baldassarre","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koray","family":"Demir","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille Pelletier","family":"Vernooy","sequence":"additional","affiliation":[{"name":"Department of Medicine St\u2010Mary's Hospital, McGill University  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume Butler","family":"Laporte","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Infectious Diseases and Medical Microbiology McGill University  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e8ve","family":"Huard","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Hepatology Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Girardin","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Nephrology Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Orlicka","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Gastroenterology Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bich Ngoc","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Division of Pathology Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Renaud","sequence":"additional","affiliation":[{"name":"Laboratory Department Division of Medical Microbiology CHU Sainte\u2010Justine  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Poirier","sequence":"additional","affiliation":[{"name":"Laboratory Department Division of Medical Microbiology CHU Sainte\u2010Justine  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Morisset","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Respirology Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Me\u2010Linh","family":"Luong","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al  Montreal Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V131277"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3062.2000.020303.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-020-00372-0"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-011-0224-6"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002191"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12531"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181eceac9"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit521"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2009.08.008"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70122-7"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0012.2004.00230.x"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2019.07.016"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw668"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2381-2386.2002"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciae321"},{"key":"e_1_2_11_17_1","unstructured":"J. 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B.Heath R.Anderson et\u00a0al. \u201cCollaborative Study to Evaluate the Proposed 1st [first] WHO International Standard for human cytomegalovirus (HCMV) for Nucleic Acid Amplification (NAT)\u2010based Assays \u201d World Health Organization Patent WHO\/BS\/10.2138 (2010)."},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01495-15"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2013.07.040"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555815974.ch3"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2017.03.008"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-018-3387-3"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-015-1741-8"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03292-15"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw370"}],"container-title":["Transplant Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.70082","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tid.70082","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.70082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T11:05:11Z","timestamp":1773054311000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tid.70082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/tid.70082"],"URL":"https:\/\/doi.org\/10.1111\/tid.70082","archive":["Portico"],"relation":{},"ISSN":["1398-2273","1399-3062"],"issn-type":[{"value":"1398-2273","type":"print"},{"value":"1399-3062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-07-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70082"}}