{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:09:24Z","timestamp":1789207764056,"version":"build-2803163510"},"reference-count":11,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Lung transplantation (LuTx) is hampered by infectious risks. Perioperative antibiotic prophylaxis (PAP) is widely used; however, real\u2010time adjustment is hindered by the timing of standard microbiology. Syndromic molecular panels offer rapid results, yet their integration into PAP strategies remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted a prospective cohort study comparing the BioFire FilmArray Pneumonia Panel Plus (PN\n                      <jats:italic>plus<\/jats:italic>\n                      ) with standard of care (SOC) on bronchoalveolar lavage (BAL) samples obtained from donors at procurement and from recipients 72\u00a0h after LuTx. Concordance between PN\n                      <jats:italic>plus<\/jats:italic>\n                      and SOC was assessed for bacterial species and antimicrobial resistance genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Fifty\u2010three donor\u2010recipient pairs were analyzed. In donor BAL, PN\n                      <jats:italic>plus<\/jats:italic>\n                      identified at least one pathogen in 67.9% (36\/53) of cases versus 63.5% (33\/53) by SOC, with a markedly shorter time to result (221\u00a0min vs. 5.3 days). Concordance between PN\n                      <jats:italic>plus<\/jats:italic>\n                      and SOC for bacterial species was substantial (Cohen's\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u00a0=\u00a00.654), particularly for\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      (Cohen's\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u00a0=\u00a00.689),\n                      <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                      (Cohen's\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u00a0=\u00a00.658), and\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      (Cohen's\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u00a0=\u00a00.731). In recipient BAL, PN\n                      <jats:italic>plus<\/jats:italic>\n                      detected pathogens in 61.5% (32\/53) compared to 47.2% (25\/53) with SOC, but overall concordance was only moderate (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u00a0=\u00a00.365). Resistance gene concordance was minimal, with PN\n                      <jats:italic>plus<\/jats:italic>\n                      often identifying additional determinants not confirmed by SOC. Viruses were detected exclusively by PN\n                      <jats:italic>plus<\/jats:italic>\n                      , while fungi were identified only by SOC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      PN\n                      <jats:italic>plus<\/jats:italic>\n                      provides rapid, clinically relevant pathogen detection in LuTx, showing substantial agreement with SOC in donor samples and offering potential to support PAP adjustment. In early post\u2010transplant recipient BAL, interpretation requires caution, and SOC remains indispensable, particularly for detecting fungi and confirming phenotypic resistance.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tid70186-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tid.70186","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T16:15:20Z","timestamp":1771604120000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Performance of the BioFire FilmArray Pneumonia Panel Plus Compared to Standard Microbiology in Lung Transplant Donor and Recipient Samples: A Prospective Cohort Study"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0383-9579","authenticated-orcid":false,"given":"Andrea","family":"Lombardi","sequence":"first","affiliation":[{"name":"SC Malattie Infettive Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"},{"name":"Dipartimento Di Fisiopatologia Medico\u2010Chirurgica e Dei Trapianti Universit\u00e0 degli Studi di Milano  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Renisi","sequence":"additional","affiliation":[{"name":"SC Malattie Infettive Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Rosso","sequence":"additional","affiliation":[{"name":"Dipartimento Di Fisiopatologia Medico\u2010Chirurgica e Dei Trapianti Universit\u00e0 degli Studi di Milano  Milan Lombardy Italy"},{"name":"SC Chirurgia Toracica e Trapianti di Polmone Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Letizia","family":"Morlacchi","sequence":"additional","affiliation":[{"name":"Dipartimento Di Fisiopatologia Medico\u2010Chirurgica e Dei Trapianti Universit\u00e0 degli Studi di Milano  Milan Lombardy Italy"},{"name":"SC Pneumologia e Fibrosi Cistica Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacopo","family":"Fumagalli","sequence":"additional","affiliation":[{"name":"SC Anestesia e Terapia Intensiva Adulti Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Cariani","sequence":"additional","affiliation":[{"name":"SC Microbiologia e Virologia Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Righi","sequence":"additional","affiliation":[{"name":"SC Chirurgia Toracica e Trapianti di Polmone Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Rossetti","sequence":"additional","affiliation":[{"name":"SC Pneumologia e Fibrosi Cistica Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7981-5226","authenticated-orcid":false,"given":"Arianna","family":"Liparoti","sequence":"additional","affiliation":[{"name":"SC Malattie Infettive Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Azzar\u00e0","sequence":"additional","affiliation":[{"name":"SC Malattie Infettive Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Mangioni","sequence":"additional","affiliation":[{"name":"SC Malattie Infettive Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Alagna","sequence":"additional","affiliation":[{"name":"SC Malattie Infettive Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Saltini","sequence":"additional","affiliation":[{"name":"SC Malattie Infettive Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Francesca","family":"Liporace","sequence":"additional","affiliation":[{"name":"SC Microbiologia e Virologia Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Abbruzzese","sequence":"additional","affiliation":[{"name":"SC Anestesia e Terapia Intensiva Adulti Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annapaola","family":"Callegaro","sequence":"additional","affiliation":[{"name":"SC Microbiologia e Virologia Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Blasi","sequence":"additional","affiliation":[{"name":"Dipartimento Di Fisiopatologia Medico\u2010Chirurgica e Dei Trapianti Universit\u00e0 degli Studi di Milano  Milan Lombardy Italy"},{"name":"SC Pneumologia e Fibrosi Cistica Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacomo","family":"Grasselli","sequence":"additional","affiliation":[{"name":"Dipartimento Di Fisiopatologia Medico\u2010Chirurgica e Dei Trapianti Universit\u00e0 degli Studi di Milano  Milan Lombardy Italy"},{"name":"SC Anestesia e Terapia Intensiva Adulti Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Nosotti","sequence":"additional","affiliation":[{"name":"Dipartimento Di Fisiopatologia Medico\u2010Chirurgica e Dei Trapianti Universit\u00e0 degli Studi di Milano  Milan Lombardy Italy"},{"name":"SC Chirurgia Toracica e Trapianti di Polmone Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Bandera","sequence":"additional","affiliation":[{"name":"SC Malattie Infettive Fondazione IRCCS Ospedale Maggiore Policlinico  Milan Lombardy Italy"},{"name":"Dipartimento Di Fisiopatologia Medico\u2010Chirurgica e Dei Trapianti Universit\u00e0 degli Studi di Milano  Milan Lombardy Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12890\u2010020\u20101151\u20109"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.14413"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2024.12.024"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.70119"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.14091"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12811"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.13725"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.13579"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.14446"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.70036"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.14448"}],"container-title":["Transplant Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.70186","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tid.70186","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.70186","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:56:34Z","timestamp":1789206994000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tid.70186"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,20]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/tid.70186"],"URL":"https:\/\/doi.org\/10.1111\/tid.70186","archive":["Portico"],"relation":{},"ISSN":["1398-2273","1399-3062"],"issn-type":[{"value":"1398-2273","type":"print"},{"value":"1399-3062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,20]]},"assertion":[{"value":"2025-12-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70186"}}