{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T06:03:47Z","timestamp":1784700227876,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pediatric data on preservation fluid cultures (PFCs) and their ability to predict preservation fluid\u2010related posttransplant infections (PF\u2010I) remain uncertain. In addition, clinical guidelines do not provide uniform recommendations regarding when to obtain PFC or how to manage positive results, particularly for children. In this study, we aimed to evaluate PFC\u2010positive status and PF\u2010I in pediatric abdominal solid organ transplant (aSOT) recipients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted a retrospective, single pediatric center study of aSOT\u2010recipients between 2012 and 2024, where PFCs were obtained routinely. Data were collected via chart review and included prespecified outcomes such as posttransplant bacterial infections, allograft rejection, unexpected return to the operating room, prolonged hospitalization, and death within 30 days posttransplant. PF\u2010I was defined as a culture\u2010concordant infection in the setting of PFC\u2010positive status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 337 aSOT recipients, PFCs were positive in 49 patients (14.5%). Coagulase\u2010negative\n                      <jats:italic>Staphylococci<\/jats:italic>\n                      and\n                      <jats:italic>Cutibacterium<\/jats:italic>\n                      spp. were the most isolated organisms (63%). Infectious complications were more frequent in patients with PFC\u2010positive status (30.6% vs. 18.4%;\n                      <jats:italic>p<\/jats:italic>\n                      = 0.049). Two PF\u2010I episodes (4.1% of PFC\u2010positive status) were identified, both in infant liver transplant recipients, involving vancomycin\u2010resistant\n                      <jats:italic>Enterococcous<\/jats:italic>\n                      and methicillin\u2010sensitive\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      . At 30 days after transplantation, a higher percentage of the PFC\u2010positive group remained hospitalized (18.4% vs. 8.7%;\n                      <jats:italic>p =<\/jats:italic>\n                      0.037), whereas of the proportion of rejection, reoperation, and mortality was similar.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>PFC\u2010positive status and PF\u2010I were uncommon in this cohort. The utility of routine bacterial PFC is primarily to identify the growth of organisms considered pathogenic and to guide preemptive antibiotic therapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tid.70282","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T05:24:58Z","timestamp":1784697898000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical Significance of Organ Preservation Fluid Cultures in Pediatric Abdominal Solid Organ Transplantation"],"prefix":"10.1111","author":[{"given":"Hassan A.","family":"Jamal","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases Department of Pediatrics Seattle Children's Hospital University of Washington School of Medicine  Seattle Washington USA"},{"name":"Unit of Infectious Diseases Faculty of Medicine Department of Pediatrics King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6026-5532","authenticated-orcid":false,"given":"Yong K.","family":"Kwon","sequence":"additional","affiliation":[{"name":"Division of Surgical Transplantation Department of Surgery University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8227-2004","authenticated-orcid":false,"given":"Pamela L.","family":"Valentino","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Department of Pediatrics University of Washington School of Medicine Seattle Children's Hospital  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0365-4267","authenticated-orcid":false,"given":"Jodi M.","family":"Smith","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Pediatrics Seattle Children's Hospital University of Washington School of Medicine  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick J.","family":"Healey","sequence":"additional","affiliation":[{"name":"Division of Transplantation Department of Surgery Seattle Children's Hospital University of Washington School of Medicine  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheyi","family":"Teoh","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Pediatrics Seattle Children's Hospital University of Washington School of Medicine  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.07.018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.14208"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40620\u2010024\u201001972\u20101"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trre.2017.11.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160701"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2009.06.036"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2019.01.070"},{"issue":"9","key":"e_1_2_9_9_1","first-page":"2248","article-title":"The Association of Organ Preservation Fluid Pathogens With Early Infection\u2010Related Events After Kidney Transplantation","volume":"12","author":"Li J.","year":"2022","journal-title":"Kidney Transplantation Diagnostics"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.14130"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/tid.13979"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofz180"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000003136"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.12.040"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010024\u201003343\u2010z"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15826"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0124483"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/595688"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20106143.2007.02112.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469\u20100691.12557"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.13507"},{"key":"e_1_2_9_22_1","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1097\/HEP.0000000000001644","article-title":"AASLD AST Practice Guideline on Adult Liver Transplantation: Candidate Evaluation","volume":"83","author":"Dove L.","year":"2026","journal-title":"Hepatology"},{"issue":"1","key":"e_1_2_9_23_1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1097\/MPG.0000000000000431","article-title":"Evaluation of the Pediatric Patient for Liver Transplantation: 2014 Practice Guideline by the American Association for the Study of Liver Diseases, American Society of Transplantation and the North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition","volume":"59","author":"Squires R. H.","year":"2014","journal-title":"Journal of Pediatric Gastroenterology and Nutrition"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2023.03.015"}],"container-title":["Transplant Infectious Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.70282","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tid.70282","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tid.70282","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T05:24:59Z","timestamp":1784697899000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tid.70282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,21]]},"references-count":23,"alternative-id":["10.1111\/tid.70282"],"URL":"https:\/\/doi.org\/10.1111\/tid.70282","archive":["Portico"],"relation":{},"ISSN":["1398-2273","1399-3062"],"issn-type":[{"value":"1398-2273","type":"print"},{"value":"1399-3062","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,21]]},"assertion":[{"value":"2026-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70282"}}