{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:07:08Z","timestamp":1789470428388,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transplant Infectious Dis"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The gut microbiota of living organ donors has been linked to transplant outcomes. However, little is known about the characteristics of the deceased donor gut microbiota or its potential impact on recipient outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed the deep intestinal microbiota from 24 deceased donors. Samples included luminal stool from the right and left colon as well as bile. Microbial composition was characterized using 16S V4 rRNA sequencing. \u03b1\u2010 and \u03b2\u2010diversity analyses were performed to compare microbial communities between donor enteric sites and against stool samples from 28 healthy community controls, 14 critically ill intensive care comparators, and 12 matched liver transplant recipients. Machine learning models and logistic regression analysis were applied to explore whether features of the donor microbiota could predict recipient post\u2010transplant complications.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Findings<\/jats:title>\n                    <jats:p>\n                      The deceased donor microbiota showed an absence of the expected compositional variability between sampling sites, with no significant differences in either \u03b1\u2010 or \u03b2\u2010diversity observed between bile, right and left colonic samples (all\n                      <jats:italic>p<\/jats:italic>\n                      &gt; 0.05). Donor samples exhibited distinct microbial profiles compared with stool from both healthy and ICU comparators, including increased abundance of potential pathogens within the Enterobacteriaceae family (all\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001). Features of the donor microbiota, particularly enrichment of Enterobacteriaceae, were associated with an increased risk of early post\u2010transplant infection in recipients (\u2264\u00a030 days;\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      =\u00a00.011).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation<\/jats:title>\n                    <jats:p>\n                      The deceased donor gut microbiota may represent a distinct microbial community with potential clinical relevance. Microbial profiling of donor enteric microbiota may help identify recipients at heightened risk of early post\u2010transplant infectious complications.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tid70322-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tid.70322","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:46:36Z","timestamp":1789469196000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Donor Microbiota Features Associated With Liver Transplant Recipient Infectious Complications: A Pilot Study Using Deep Intestinal Sampling During Liver Procurement"],"prefix":"10.1111","author":[{"given":"Olivia C.","family":"Smibert","sequence":"first","affiliation":[{"name":"Sir Peter MacCallum Department of Oncology University of Melbourne  Melbourne Australia"},{"name":"Department of Infectious Diseases Peter MacCallum Cancer Centre  Melbourne Australia"},{"name":"National Centre for Infections in Cancer Peter MacCallum Cancer Centre  Melbourne Australia"},{"name":"Department of Infectious Diseases and Immunology Austin Health  Heidelberg Australia"},{"name":"Department of Infectious Diseases University of Melbourne The Peter Doherty Institute for Infection and Immunity  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geraldine","family":"Kong","sequence":"additional","affiliation":[{"name":"Centre for Pathogen Genomics Department of Microbiology and Immunology The University of Melbourne The Peter Doherty Institute for Infection and Immunity  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate A.","family":"Markey","sequence":"additional","affiliation":[{"name":"Translational Science and Therapeutics Division Fred Hutchinson Cancer Center (FHCC)  Seattle Washington USA"},{"name":"Department of Medicine University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise M.","family":"Judd","sequence":"additional","affiliation":[{"name":"Centre for Pathogen Genomics Department of Microbiology and Immunology The University of Melbourne The Peter Doherty Institute for Infection and Immunity  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Howden","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology Austin Health  Heidelberg Australia"},{"name":"Centre for Pathogen Genomics Department of Microbiology and Immunology The University of Melbourne The Peter Doherty Institute for Infection and Immunity  Melbourne Australia"},{"name":"Microbiological Diagnostic Unit Public Health Laboratory Department of Microbiology and Immunology The University of Melbourne at the Peter Doherty Institute for Infection and Immunity  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P.","family":"Stinear","sequence":"additional","affiliation":[{"name":"Centre for Pathogen Genomics Department of Microbiology and Immunology The University of Melbourne The Peter Doherty Institute for Infection and Immunity  Melbourne Australia"},{"name":"Microbiological Diagnostic Unit Public Health Laboratory Department of Microbiology and Immunology The University of Melbourne at the Peter Doherty Institute for Infection and Immunity  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology Austin Health  Heidelberg Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Varun J.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Surgery (Austin Health) Melbourne Medical School The University of Melbourne  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohit","family":"D'Costa","sequence":"additional","affiliation":[{"name":"DonateLife Victoria  Carlton Australia"},{"name":"Department of Intensive Care Medicine Melbourne Health  Melbourne Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Sinclair","sequence":"additional","affiliation":[{"name":"Liver Transplant Unit, Austin Health  Heidelberg Australia"},{"name":"School of Medicine Dentistry and Health Sciences, The University of Melbourne  Carlton Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avik","family":"Majumdar","sequence":"additional","affiliation":[{"name":"Liver Transplant Unit, Austin Health  Heidelberg Australia"},{"name":"School of Medicine Dentistry and Health Sciences, The University of Melbourne  Carlton Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham","family":"Starkey","sequence":"additional","affiliation":[{"name":"Liver Transplant Unit, Austin Health  Heidelberg Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Testro","sequence":"additional","affiliation":[{"name":"Liver Transplant Unit, Austin Health  Heidelberg Australia"},{"name":"School of Medicine Dentistry and Health Sciences, The University of Melbourne  Carlton Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason 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