{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:05:45Z","timestamp":1792717545447,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,3,31]],"date-time":"2015-03-31T00:00:00Z","timestamp":1427760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Providing adequate transfusion support for alloimmunised patients for whom antigen negative blood is not readily available is hampered by the risk of a haemolytic reaction. The monocyte monolayer assay (<jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content>) has shown good correlation between the antibody clinical significance and the fate of antigen positive blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>From 2006 to 2013, the clinical significance of red cell alloantibodies produced by 61 patients was evaluated using a <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content>; and antigen positive blood offering the best survival advantage was selected for transfusion following a secondary <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content> crossmatch. Post\u2010transfusion, patients were evaluated for clinical signs of haemolysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, 19 of 61 (31\u00b71%) of our antibodies were potentially clinically significant, with a monocyte index (<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>)\u2009&gt;\u20095%. There was no correlation between the clinical significance as showed by the <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content>, and the specificity of the antibody or the strength of reactivity at antihuman globulin (<jats:styled-content style=\"fixed-case\">AHG<\/jats:styled-content>) phase. Using the <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content> as a secondary crossmatch method, 31 alloimmunised patients (including: eight anti\u2010<jats:styled-content style=\"fixed-case\">hr<jats:sup>B<\/jats:sup><\/jats:styled-content>, four anti\u2010Yt<jats:sup>a<\/jats:sup>, one each anti\u2010Rg1, \u2010Co<jats:sup>a<\/jats:sup>, Er<jats:sup>a<\/jats:sup>, Le<jats:sup>b<\/jats:sup>, \u2010<jats:styled-content style=\"fixed-case\">LW<\/jats:styled-content>, \u2010Sl1) received 103 antigen positive blood units with no clinical sign of a post\u2010transfusion reaction. For three patients (one each anti\u2010Jo<jats:sup>a<\/jats:sup>, \u2010<jats:styled-content style=\"fixed-case\">AnWj<\/jats:styled-content>, unidentified \u2018<jats:styled-content style=\"fixed-case\">HTLA<\/jats:styled-content>\u2019), initial <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content> was performed as part of an investigation of a suspected haemolytic reaction. In each case, the <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content> accurately identified the unit responsible for the reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Used as a crossmatch surrogate, the <jats:styled-content style=\"fixed-case\">MMA<\/jats:styled-content> provided valuable information in the decision of transfusing antigen positive blood to alloimmunised patients, avoiding delay because of the search of rare antigen negative units.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12189","type":"journal-article","created":{"date-parts":[[2015,4,2]],"date-time":"2015-04-02T19:44:25Z","timestamp":1428003865000},"page":"92-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Successful transfusion of antigen positive blood to alloimmunised patients using a monocyte monolayer assay"],"prefix":"10.1111","volume":"25","author":[{"given":"G. 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