{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:02:03Z","timestamp":1761541323955},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,5,30]],"date-time":"2016-05-30T00:00:00Z","timestamp":1464566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"LFB Biomedicaments"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Patients with bone marrow failure secondary to chemotherapy often develop thrombocytopenia and require platelet transfusion. Fibrinogen plays an important role in platelet aggregation and the establishment of the primary haemostatic plug.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To compare the effects of <jats:italic>in vivo<\/jats:italic> platelet transfusion on clot firmness in thrombocytopenic patients with <jats:italic>in vitro<\/jats:italic>\u2010performed fibrinogen concentrate substitution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Thirty patients with haematological malignancy admitted for platelet transfusion were included. Haemostatic effects from platelet transfusion and <jats:italic>ex vivo<\/jats:italic> addition of fibrinogen concentrate at three different doses were evaluated by thromboelastometry, with clot firmness as the primary endpoint (<jats:styled-content style=\"fixed-case\">A30 ExTEM<\/jats:styled-content> assay). Secondary endpoints were other thromboelastometry parameters, thrombin generation parameters, activated partial thromboplastin time (<jats:styled-content style=\"fixed-case\">APTT<\/jats:styled-content>), prothrombin time <jats:styled-content style=\"fixed-case\">PT<\/jats:styled-content>, fibrinogen and factor <jats:styled-content style=\"fixed-case\">XIII<\/jats:styled-content> levels and a clinical bleeding score.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty patients (66%) had clinical bleeding signs by prior to transfusion. Platelets increased from 17 (range, 1\u2013109) to 40 (range 2\u2013139)\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\u2009L<jats:sup>\u22121<\/jats:sup> following transfusion, with a median corrected count increment of 16\u00b77 (range, 0\u00b78\u201343\u00b75). The <jats:styled-content style=\"fixed-case\">A30<\/jats:styled-content> value increased significantly by platelet transfusion from 35\u2009\u00b1\u200911 to 47\u2009\u00b1\u200910\u2009mm, with no changes in thrombin generation. Fibrinogen concentrate dose\u2010dependently increased A 30 (to 43\u2009\u00b1\u200910, 49\u2009\u00b1\u20099 and 50\u2009\u00b1\u20099\u2009mm, respectively) and reduced parameters of thrombin generation at high doses. Platelet transfusion, together with fibrinogen concentrate, further increased clot firmness. <jats:styled-content style=\"fixed-case\">APTT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PT<\/jats:styled-content> were within normal range, whereas fibrinogen levels were slightly elevated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Fibrinogen concentrate increased clot firmness to the same degree as platelet transfusion in patients with low platelet count requiring platelet transfusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12323","type":"journal-article","created":{"date-parts":[[2016,5,30]],"date-time":"2016-05-30T11:23:10Z","timestamp":1464607390000},"page":"291-296","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Fibrinogen concentrate improves clot strength in patients with haematological malignancies requiring platelet transfusion"],"prefix":"10.1111","volume":"26","author":[{"given":"H.","family":"Munk\u2010Andersen","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology Aarhus University Hospital  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Schenk","sequence":"additional","affiliation":[{"name":"Department for General and Surgical Intensive Care Medicine Medical University Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.H.","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Haematology Aarhus University Hospital  Aarhus Denmark"},{"name":"Centre for Haemophilia and Thrombosis, Department of Clinical Biochemistry Aarhus University Hospital  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Fries","sequence":"additional","affiliation":[{"name":"Department for General and Surgical Intensive Care Medicine Medical University Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Fenger\u2010Eriksen","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Aarhus University Hospital  Aarhus Denmark"},{"name":"Centre for Haemophilia and Thrombosis, Department of Clinical Biochemistry Aarhus University Hospital  Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2007.02297.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aes096"},{"key":"e_1_2_7_4_1","first-page":"Cd004269","article-title":"Prophylactic platelet transfusion for prevention of bleeding in patients with haematological disorders after chemotherapy and stem cell transplantation","volume":"5","author":"Estcourt L.","year":"2012","journal-title":"The Cochrane Database 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