{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:15:00Z","timestamp":1783466100972,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T00:00:00Z","timestamp":1514332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Alloantibodies against human neutrophil antigens (HNA) are associated with a variety of clinical conditions. Over the past decade, the allelic and genotypic frequencies of the five HNA systems have been evaluated. Although the HNA system is less polymorphic than human leukocyte antigens (HLA), significant differences in the genotypic and allele frequencies still exist in different populations, even those living in close proximity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To delineate HNA genotypic and allele frequencies to provide vital information on estimating the risk of HNA\u2010associated diseases for our local population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using a validated, in\u2010house\u2010developed assay, genotyping for HNA\u20101, HNA\u20103, HLA\u20104 and HNA\u20105 was performed on 300 samples from Chinese blood donors from Hong Kong. In addition, the frequency of the HNA\u20102 c.843A\u2009&gt;\u2009T allele was also determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The allele frequencies of HNA\u20101a, \u20101b and \u20101c alleles were 67\u00b78, 31\u00b75 and 0%, respectively, whereas the frequencies of HNA\u20103a and HNA\u20103b were 71\u00b70 and 29\u00b70%, respectively. The frequencies of HNA\u20104a and \u20104b alleles were 99\u00b75 and 0\u00b75%, respectively, and for HNA\u20105a and \u20105b, alleles were 85\u00b72 and 14\u00b78%, respectively. Homozygotes for the HNA\u20102 c.843\u2009TT variant were absent in our population, whereas only &lt;4% of the population were c.843AT heterozygote carriers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This is the first study to define HNA genotype and allele frequencies using a validated modified in\u2010house PCR\u2010SSP method in the Hong Kong Chinese blood donor population. Our approach provides a cost\u2010effective assay for conducting routine HNA typing and facilitates the incorporation of these assays into routine clinical service. Our results are comparable with those reported in the Guangzhou Chinese population, but the allele frequencies in our Hong Kong Chinese population are significantly different from the reported European frequencies, confirming that a geographical difference exists for HNA allele frequencies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12494","type":"journal-article","created":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T05:52:38Z","timestamp":1514353958000},"page":"310-318","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A study of human neutrophil antigen genotype frequencies in Hong Kong"],"prefix":"10.1111","volume":"28","author":[{"given":"K.","family":"Tam","sequence":"first","affiliation":[{"name":"Division of Transplantation and Immunogenetics, Department of Pathology Queen Mary Hospital  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Tang","sequence":"additional","affiliation":[{"name":"Division of Transplantation and Immunogenetics, Department of Pathology Queen Mary Hospital  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ho","sequence":"additional","affiliation":[{"name":"Division of Transplantation and Immunogenetics, Department of Pathology Queen Mary Hospital  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8534-7983","authenticated-orcid":false,"given":"W.","family":"Yeung","sequence":"additional","affiliation":[{"name":"Division of Transplantation and Immunogenetics, Department of Pathology Queen Mary Hospital  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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