{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:21Z","timestamp":1787016201875,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T00:00:00Z","timestamp":1536710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2019,4]]},"abstract":"SUMMARY<\/jats:title>\n \n Objectives<\/jats:title>\n To investigate whether microRNAs can serve as biomarkers for iron status in blood donors.<\/jats:p>\n <\/jats:sec>\n \n Background<\/jats:title>\n Serum ferritin is a widely used biochemical test for detecting iron deficiency, but it has its limitations. Certain microRNAs (miRNAs) reportedly have a role in regulating iron homeostasis. Circulating miRNAs have been reported as potential biomarkers for various conditions but have not yet been studied in iron deficiency.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Participating blood donors were divided into two groups: high ferritin (HF) (>150 \u00b5g L\n \u22121<\/jats:sup>\n ) and low ferritin (LF) (<15 \u00b5g L\n \u22121<\/jats:sup>\n ). MiRNA analysis was performed by an miRNA profiling service (Exiqon) using commercial qPCR assays. The study had two phases: (i) a pilot study (20 participants) where 179 miRNAs were analysed and (ii) a confirmation study (50 participants) of 13 selected miRNAs.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Mean serum ferritin was 13\u00b78 \u00b5g L\n \u22121<\/jats:sup>\n in the LF arm compared to 231 \u00b5g L\n \u22121<\/jats:sup>\n in the HF group (\n P<\/jats:italic>\n < 0\u00b7001). Hepcidin plasma levels were higher in the HF arm (\n P<\/jats:italic>\n < 0\u00b7001), whereas soluble transferrin receptor 1 was higher in the LF group (\n P<\/jats:italic>\n < 0\u00b7001). In the pilot study, samples did not separate according to study group on unsupervised analysis. When directly comparing HF vs LF groups, 17 miRNAs were differentially expressed (\n P<\/jats:italic>\n < 0\u00b705,\n t<\/jats:italic>\n \u2010test) but did not pass correction for multiple testing. The confirmation study of 13 selected miRNAs verified these findings as no miRNA was significantly different between the study groups.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n In this study, circulating plasma miRNAs did not emerge as promising biomarkers for iron status in healthy individuals. However, in the future, alternative detection methods such as next\u2010generation sequencing might indicate miRNAs that correlate with iron stores.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/tme.12554","type":"journal-article","created":{"date-parts":[[2018,9,13]],"date-time":"2018-09-13T01:43:23Z","timestamp":1536803003000},"page":"52-58","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Circulating plasma microRNAs as biomarkers for iron status in blood donors"],"prefix":"10.1111","volume":"29","author":[{"given":"M. G.","family":"Gunnarsdottir","sequence":"first","affiliation":[{"name":"Faculty of Biomedical Sciences University of Iceland Reykjavik Iceland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Jonsson","sequence":"additional","affiliation":[{"name":"Blood Bank Landspitali National University Hospital Reykjavik Iceland"},{"name":"Faculty of Medicine University of Iceland Reykjavik Iceland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"Halldorsdottir","sequence":"additional","affiliation":[{"name":"Blood Bank Landspitali National University Hospital Reykjavik Iceland"},{"name":"Faculty of Medicine University of Iceland Reykjavik Iceland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03956.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2012.09.015"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/79.3.437"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12061"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03401.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI44883"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-292011"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2891-9-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.082701.108"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1651"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804549105"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2010.01427.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2008.10.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-010-0849-4"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2015.03.011"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1800"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12260"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.2.565S"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-10-8"},{"key":"e_1_2_7_21_1","unstructured":"World Health Organization(2001).Iron Deficiency Anaemia. Assessment Prevention and Control. A guide for programme managers. URLhttp:\/\/www.who.int\/nutrition\/publications\/micronutrients\/anaemia_iron_deficiency\/WHO_NHD_01.3\/en\/(Accessed 30\/09\/17)."},{"key":"e_1_2_7_22_1","volume-title":"Assessing the Iron Status of Populations","author":"World Health Organization","year":"2007"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.577387"}],"container-title":["Transfusion Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftme.12554","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tme.12554","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tme.12554","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tme.12554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T07:24:13Z","timestamp":1694330653000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tme.12554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,12]]},"references-count":22,"journal-issue":{"issue":"S1","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1111\/tme.12554"],"URL":"https:\/\/doi.org\/10.1111\/tme.12554","archive":["Portico"],"relation":{},"ISSN":["0958-7578","1365-3148"],"issn-type":[{"value":"0958-7578","type":"print"},{"value":"1365-3148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,12]]},"assertion":[{"value":"2017-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}