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Such a situation, combined with a recent outbreak of tick\u2010borne Rocky Mountain spotted fever in the S\u00e3o Paulo state demonstrates the increasing role of ticks as arthropod vectors in Brazil.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>For the purpose of the study, 452 blood donors from Ribeir\u00e3o Preto city, S\u00e3o Paulo state were tested using anti\u2010<jats:italic>Borrelia<\/jats:italic> immunoglobulin G (IgG) assay. The positive results were also confirmed by Western blot for anti\u2010borrelia IgM\/IgG.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The anti\u2010<jats:italic>Borrelia<\/jats:italic> IgG enzyme\u2010linked immunosorbent assay (ELISA) showed nine positive and nine borderline reactive samples, giving a total seroprevalence of 2\u00b70% of anti\u2010<jats:italic>Borrelia<\/jats:italic> IgG among Brazilian blood donors. The confirmation of the seropositive and borderline samples by Borrelia Western blot was demonstrated by IgG\u2010positive results in 16 samples (a seroprevalence of 3.5%). Anti\u2010<jats:italic>Borrelia<\/jats:italic> IgM antibodies were also detected in one sample.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results demonstrate that <jats:italic>Borrelia<\/jats:italic>\u2010like spirochetes may be circulating among blood donors from the S\u00e3o Paulo State and that the ticks have an important epidemiological role as vectors of bacterial infections in this Brazilian region. These results not only alert us to possible actions that might be undertaken in order to completely characterise the aetiological agents of Lyme\u2010like syndromes in Brazil but also the possible impact that these bacterial agents might have on haemotherapy practices.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12627","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T08:06:28Z","timestamp":1567152388000},"page":"358-363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Serological evidence of <i>Borrelia<\/i> circulation among blood donors in the S\u00e3o Paulo state, Brazil"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0805-6140","authenticated-orcid":false,"given":"S. N.","family":"Slavov","sequence":"first","affiliation":[{"name":"Blood Center of Ribeir\u00e3o Preto, Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Department of Internal Medicine, Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. S.","family":"Christova","sequence":"additional","affiliation":[{"name":"Department of Microbiology National Center of Infectious and Parasitic Diseases  Sofia Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. R.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Blood Center of Ribeir\u00e3o Preto, Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. S.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Blood Center of Ribeir\u00e3o Preto, Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. L.","family":"Bianquini","sequence":"additional","affiliation":[{"name":"Blood Center of Ribeir\u00e3o Preto, Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Hespanhol","sequence":"additional","affiliation":[{"name":"Blood Center of Ribeir\u00e3o Preto, Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. T.","family":"Covas","sequence":"additional","affiliation":[{"name":"Blood Center of Ribeir\u00e3o Preto, Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kashima","sequence":"additional","affiliation":[{"name":"Blood Center of Ribeir\u00e3o Preto, Faculty of Medicine of Ribeir\u00e3o Preto University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,8,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit235"},{"key":"e_1_2_7_3_1","unstructured":"Brazil M. of H. (2018a). Confirmed cases of spotted fever. Brazil Major Regions and Federated Units. 2000 to 2019. 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