{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T13:04:52Z","timestamp":1769691892034,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,11,6]],"date-time":"2019-11-06T00:00:00Z","timestamp":1572998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to assess the efficacy of the INTERCEPT\u2122 Blood System [amotosalen\/ultraviolet A (UVA) light] to reduce the risk of Middle East respiratory syndrome\u2010Coronavirus (MERS\u2010CoV) transmission by human platelet concentrates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Since 2012, more than 2425 MERS\u2010CoV human cases have been reported in 27 countries. The infection causes acute respiratory disease, which was responsible for 838 deaths in these countries, mainly in Saudi Arabia. Viral genomic RNA was detected in whole blood, serum and plasma of infected patients, raising concerns of the safety of blood supplies, especially in endemic areas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four apheresis platelet units in 100% plasma were inoculated with a clinical MERS\u2010CoV isolate. Spiked units were then treated with amotosalen\/UVA to inactivate MERS\u2010CoV. Infectious and genomic viral titres were  quantified by plaque assay and quantitative real\u2010time  reverse transcription polymerase chain reaction (RT\u2010qPCR). Inactivated samples were successively passaged thrice on Vero E6 cells to exclude the presence of residual replication\u2010competent viral particles in inactivated platelets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Complete inactivation of MERS\u2010CoV in spiked platelet units was achieved by treatment with Amotosalen\/UVA light with a mean log reduction of 4\u00b748\u2009\u00b1\u20090\u00b73. Passaging of the  inactivated samples in Vero E6 showed no viral replication even after nine\u2009days of incubation and three passages. Viral genomic RNA titration in inactivated samples showed titres comparable to those in pre\u2010treatment samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Amotosalen and UVA light treatment of MERS\u2010CoV\u2010spiked platelet concentrates efficiently and completely inactivated MERS\u2010CoV infectivity (&gt;4 logs), suggesting that such treatment could minimise the risk of transfusion\u2010related MERS\u2010CoV transmission.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12638","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T05:14:33Z","timestamp":1573103673000},"page":"434-441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Amotosalen and ultraviolet A light efficiently inactivate MERS\u2010coronavirus in human platelet concentrates"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8471-7011","authenticated-orcid":false,"given":"A. M.","family":"Hashem","sequence":"first","affiliation":[{"name":"Special Infectious Agents Unit King Fahd Medical Research Center, King Abdulaziz University  Jeddah Saudi Arabia"},{"name":"Vaccines and Immunotherapy Unit King Fahd Medical Research Center, King Abdulaziz University  Jeddah Saudi Arabia"},{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine, King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Special Infectious Agents Unit King Fahd Medical Research Center, King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Tolah","sequence":"additional","affiliation":[{"name":"Special Infectious Agents Unit King Fahd Medical Research Center, King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Alsaadi","sequence":"additional","affiliation":[{"name":"Special Infectious Agents Unit King Fahd Medical Research Center, King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Abunada","sequence":"additional","affiliation":[{"name":"Cerus Europe B.V  Amersfoort The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. A.","family":"Damanhouri","sequence":"additional","affiliation":[{"name":"Department of Hematology, Faculty of Medicine King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. A.","family":"El\u2010Kafrawy","sequence":"additional","affiliation":[{"name":"Special Infectious Agents Unit King Fahd Medical Research Center, King Abdulaziz University  Jeddah Saudi Arabia"},{"name":"Department of Medical Laboratory Technology Faculty of Applied Medical Sciences, King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Picard\u2010Maureau","sequence":"additional","affiliation":[{"name":"Cerus Europe B.V  Amersfoort The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. I.","family":"Azhar","sequence":"additional","affiliation":[{"name":"Special Infectious Agents Unit King Fahd Medical Research Center, King Abdulaziz University  Jeddah Saudi Arabia"},{"name":"Department of Medical Laboratory Technology Faculty of Applied Medical Sciences, King Abdulaziz University  Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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