{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:05:53Z","timestamp":1747188353925,"version":"3.40.5"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201000424\u201016"],"award-info":[{"award-number":["APQ\u201000424\u201016"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The present study aimed to develop strategies for genotyping <jats:italic>DO*HY<\/jats:italic> (Dombrock system) and <jats:italic>DI*A\/DI*B<\/jats:italic> (Diego system) alleles and to evaluate the impact of genomic and self\u2010declared ancestry on rare donor screening in admixed populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>The antigens Hy and Di<jats:sup>b<\/jats:sup> demonstrate clinical importance. The lack of antisera for the serological evaluation of these antigens makes it necessary to develop molecular methods. In addition, considering that some rare red blood cell phenotypes present differences in frequency between ethnic groups, it is important to assess the applicability of self\u2010declared ancestry in the search for rare donors in admixed populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>DO*HY<\/jats:italic> and <jats:italic>DI*A\/DI*B<\/jats:italic> genotyping based on real\u2010time polymerase chain reaction (PCR) was standardised. A total of 457 blood donors clustered by self\u2010defined skin colour\/race categories were genotyped. Furthermore, individual genomic ancestry was used in the analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The assays developed are reproducible and provide satisfactory results even at low concentrations of DNA, which make them useful in situations where the DNA is scarce, such as dried blood spots on filter paper, or when screening for pooled samples. No significant difference was observed in the frequencies of the <jats:italic>DI*A<\/jats:italic>, <jats:italic>DI*B<\/jats:italic> and <jats:italic>DO*HY<\/jats:italic>, comparing the self\u2010declared White (branco) donors with those who are Black (preto) and Brown (pardo).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Real\u2010time PCR, especially using pooled samples, is a promising strategy to screen rare blood donors. Although both self\u2010reported race\/colour and some blood group phenotypes are associated with ancestry, the results point to a greater complexity in the application of self\u2010declared race\/colour in the screening of rare donors in admixed populations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12653","type":"journal-article","created":{"date-parts":[[2019,12,10]],"date-time":"2019-12-10T02:27:31Z","timestamp":1575944851000},"page":"148-156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The screening of rare blood donors in a highly admixed population: A new approach for Holley and Diego genotyping and impact of genomic and self\u2010reported ancestry"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5070-7472","authenticated-orcid":false,"given":"Amanda A.","family":"Muniz","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Hemominas  Belo Horizonte Brazil"},{"name":"Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0878-2479","authenticated-orcid":false,"given":"Ad\u00e3o R.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemominas  Belo Horizonte Brazil"},{"name":"Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5346-7793","authenticated-orcid":false,"given":"Isabela A.","family":"Ferraz","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemominas  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2506-8450","authenticated-orcid":false,"given":"Marina L.","family":"Martins","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemominas  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1925-4161","authenticated-orcid":false,"given":"Mariana M.","family":"Godin","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemominas  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9445-7911","authenticated-orcid":false,"given":"Luciana C.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemominas  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7484-1684","authenticated-orcid":false,"given":"Luci M. 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