{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T10:26:50Z","timestamp":1785493610682,"version":"3.56.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T00:00:00Z","timestamp":1576540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD0500300"],"award-info":[{"award-number":["2017YFD0500300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aimed to identify the emerging\/reemerging pathogens in blood donation samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>A metagenomic analysis has previously been used to look for pathogens but in this study, the relationship with aminotransferase (ALT) is described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods\/Materials<\/jats:title><jats:p>Excluding samples reactive to hepatitis B virus, hepatitis C virus, human immunodeficiency syndrome virus or syphilis and plasma samples were stratified into three groups of ALT levels (IU\/L): A\u2009\u2264\u200950, B 51 to 69 and C\u2009\u2265\u200970, respectively. Each group was mixed in a pool of 100 samples, from which DNA and cDNA libraries were established for next generation sequencing and analysis. Pathogens of interest were identified by immunoassays, nested\u2010polymerase chain reaction, phylogenetic analysis and pathogen detection in follow\u2010up donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Several new or reemerging transfusion\u2010transmitted pathogens were identified; <jats:italic>Streptococcus suis<\/jats:italic>, <jats:italic>Babesia species<\/jats:italic> and <jats:italic>Toxoplasma gondii<\/jats:italic> were found in the three ALT groups, Epstein\u2010Barr virus (EBV) only in group C. Ten <jats:italic>S. suis<\/jats:italic> nucleic acid positive samples were detected, all closely phylogenetically related to reference strains. A donor in group A carried both <jats:italic>S. suis<\/jats:italic> genome and specific IgM in follow\u2010up samples. This strain was identified as nontoxic <jats:italic>S. suis<\/jats:italic>. Five samples contained a short fragment of <jats:italic>Babesia species<\/jats:italic> SpeI\u2010AvaI gene, while <jats:italic>T. gondii<\/jats:italic> was identified in 20 samples as a short fragment of 18S rDNA gene. In group C, two samples contained EBV genome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Blood donations that contained <jats:italic>S. suis, Babesia species and T. gondii<\/jats:italic> sequences might represent potential transfusion risks. EBV, a potential cause of elevated ALT, was detected. Metagenomic analysis might be a useful technology for monitoring blood safety.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12657","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T08:03:51Z","timestamp":1576569831000},"page":"61-69","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Metagenomic analysis of potential pathogens from blood donors in Guangzhou, China"],"prefix":"10.1111","volume":"30","author":[{"given":"Lei","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology Southern Medical University  Guangzhou China"},{"name":"Institute of Blood Transfusion Chinese Academy of Medical Sciences  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Rong","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, School of 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