{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T01:25:05Z","timestamp":1788312305596,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,6,3]],"date-time":"2020-06-03T00:00:00Z","timestamp":1591142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Intramural Research Project funded by Sanjay Gandhi Postgraduate Institute of Medical Sciences"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>A molecular analysis of serologically RhD variant samples was conducted to find the incidence of various D variants in our blood donor population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Determining a blood donor's RhD phenotype and genotype is important as transfusion of units with a weak D or partial D phenotype can result in immunisation of the recipients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Samples with discrepant D and weak D phenotypes identified on testing with at least five different monoclonal anti\u2010D antisera were considered serological RhD variant and subjected to molecular testing (Massarray kit, Agena Bioscience, San Diego) for variant<jats:italic>RHD<\/jats:italic>gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 39 samples, including 19 RhD discrepant samples and 20 weak D samples, were identified as serological RhD variant from a total of 4386 samples. Thirteen (13\/39) samples carried variants leading to weak D phenotype, and eight samples had variants leading to partial D categories. Seven samples (7) could not be characterised, whereas 11 samples were identified as Rh negative (<jats:italic>RHD*01N.01<\/jats:italic>) after molecular testing. Overall incidence of D variants in the study population was 0.48%.<jats:italic>RHD*weak D type 1<\/jats:italic>(5, 0.1%) and<jats:italic>RHD*DFR1<\/jats:italic>(5, 1%) were the most common variants identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Few samples with weak reaction on serological testing were found to be partial D variant and vice versa. Donor centres should develop a protocol for genotyping of samples with aberrant results on serological testing for assessing the actual RhD status of an individual as results of serological testing may be misleading.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12690","type":"journal-article","created":{"date-parts":[[2020,6,3]],"date-time":"2020-06-03T07:33:02Z","timestamp":1591169582000},"page":"295-303","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular characterisation of<scp>RhD<\/scp>variants in North Indian blood donor population"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1807-2390","authenticated-orcid":false,"given":"Dheeraj","family":"Khetan","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine Sanjay Gandhi Postgraduate Institute of Medical Sciences Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anviti","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Sanjay Gandhi Postgraduate Institute of Medical Sciences Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra K.","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Sanjay Gandhi Postgraduate Institute of Medical Sciences Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jai Shankar","family":"Shukla","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Sanjay Gandhi Postgraduate Institute of Medical Sciences Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41010045.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12275"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12941"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.6.843"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-419"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-2-10"},{"issue":"2","key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1111\/trf.12145","article-title":"D category IV: a group of clinically relevant and phylogenetically diverse partial D","volume":"53","author":"Von Zabern I","year":"2013","journal-title":"Transfusion"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0041-1132.2004.04211.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0041-1132.2005.04256.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00579.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0041-1132.2005.05001.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00625.x"},{"key":"e_1_2_8_14_1","unstructured":"Drugs and cosmetics Rules1945 Schedule F Part XII B.Requirements for the functioning and operation of a blood bank and\/or for preparation of blood components. 302\u2013321.https:\/\/cdsco.gov.in\/opencms\/export\/sites\/CDSCO_WEB\/Pdf-documents\/Ethics-Committee\/Guideline-Document\/Drugs_CosmeticsAct1940_Rules1945.pdf. 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