{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:25:40Z","timestamp":1791383140004,"version":"4.3.3"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T00:00:00Z","timestamp":1595808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005930","name":"ASCRS Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005930","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["no. 2016A030313123"],"award-info":[{"award-number":["no. 2016A030313123"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["no. 2016A030313124"],"award-info":[{"award-number":["no. 2016A030313124"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81601451"],"award-info":[{"award-number":["81601451"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970169"],"award-info":[{"award-number":["81970169"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In the present study, we asked whether anti\u2010CD36 antibodies impair the maturation of erythropoietic stem cells to mature red blood cells (RBCs), leading to anaemia and hydrops fetalis (HF).<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Recent studies have shown the importance of anti\u2010CD36 antibodies in the development of Fetal\/Neonatal Alloimmune Thrombocytopenia (FNAIT). In comparison to other types of antibody\u2010mediated FNAIT, anti\u2010CD36 antibodies are frequently associated with anaemia and HF. As mature RBCs do not express CD36, the reason for this phenomenon is currently not fully understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>A case of FNAIT with signs of HF was characterised in this study. Maternal anti\u2010CD36 antibodies were isolated by an absorption\/elution approach. We cultured haematopoietic stem cells (HSCs) with purified anti\u2010CD36 antibodies, and the formation of burst\u2010forming unit\u2010erythroid and colony\u2010forming unit\u2010erythroid (CFU\u2010E\/BFU\u2010E) cells was analysed. Apoptosis of HSCs was also investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Analysis of the mother showed type\u20101 CD36 deficiency. Anti\u2010CD36 antibodies were found in maternal serum, as well as on fetal platelets, by ELISA, and the specificity of these antibodies was further substantiated by flow cytometry. In comparison to control IgG, incubation of HSCs with purified anti\u2010CD36 antibodies led to a significant reduction in CFU\u2010E\/BFU\u2010E cell formation, and this result was associated with an increased number of apoptotic CD34+ erythroid\/myeloid precursor cells. Administration of intra\u2010uterine transfusion with washed RBCs was effective in improving fetal anaemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Anti\u2010CD36 antibodies may cause anaemia and trigger HF through apoptosis of CD34+ erythroid\/myeloid precursor cells. However, the contribution of other cells must also be taken into account.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12705","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T06:02:38Z","timestamp":1595916158000},"page":"361-368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Hydrops fetalis associated with <scp>anti\u2010CD36<\/scp> antibodies in fetal and neonatal alloimmune thrombocytopenia: Possible underlying mechanism"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6512-4085","authenticated-orcid":false,"given":"Yongbin","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology Southern Medical University  Guangzhou Guangdong 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