{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T14:56:32Z","timestamp":1790088992079,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T00:00:00Z","timestamp":1600905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to assess the frequency distribution of of ABO haemolytic disease of newborn (ABO\u2010HDN) and to know the predictive value of immunohaematological tests in identifying at risk neonates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>ABO incompatibility, although a common cause of haemolytic disease of newborn, has several unaddressed issues related to it.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>A prospective study over 20\u2009months was carried out in a tertiary care centre in South India. Blood grouping, Direct Antiglobulin test (DAT) and elution studies were performed on neonatal samples, whereas blood grouping, antibody screening and antibody titration were performed on maternal samples. In suspected cases, ABO\u2010HDN was diagnosed after excluding other possible causes for haemolysis. The laboratory results were correlated with the clinical details to assess the predictive value of the tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the total 2856 pregnancies, 34% had ABO incompatibility. On testing with columnagglutination test (CAT), the overall DAT positivity and that among ABO\u2010incompatible cases were 3.8% and 11.2%, respectively,) whereas by conventinal tube technique (CTT) it was 0.6% and 2.4% respectively. CAT was found to have higher sensitivity, and the predictive value was higher for CTT. Maternal IgG titre showed a positive linear relationship with the DAT strength and the rise in indirect bilirubin levels. The positive predictive value of combination of tests such as DAT, elution and titation was 94.12%, which was much higher than that of the individual tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>DAT positivity is a predictor of early rise in serum bilirubin level, and a combination of tests has a better predictive value than individual tests towards development of clinically significant hyperbilirubinemia in ABO\u2010HDN.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12718","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T11:23:08Z","timestamp":1600946588000},"page":"30-35","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Clinical Implication of Immunohaematological Tests in <scp>ABO<\/scp> haemolytic disease of newborn: Revisiting an old disease"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2589-8315","authenticated-orcid":false,"given":"Soumya","family":"Das","sequence":"first","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Manipal Academy of Higher Education, Manipal. Karnataka. India  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9796-2326","authenticated-orcid":false,"given":"Shamee","family":"Shastry","sequence":"additional","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Manipal Academy of Higher Education, Manipal. Karnataka. India  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5462-5677","authenticated-orcid":false,"given":"P Kalyana","family":"Chakravarthy","sequence":"additional","affiliation":[{"name":"Department of Public Health Dentistry, Manipal College of Dental Sciences Manipal Academy of Higher Education  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poornima B","family":"Baliga","sequence":"additional","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Manipal Academy of Higher Education, Manipal. Karnataka. India  Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90466-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","unstructured":"Bucher KA Patterson AM Jr Elston RC Jones CA Kirkman HN Jr.Racial difference in incidence of ABO hemolytic disease.Am J Public Health1976;66(9):854\u2010858.https:\/\/doi.org\/10.2105\/ajph.66.9.854","DOI":"10.2105\/AJPH.66.9.854"},{"issue":"2","key":"e_1_2_7_4_1","first-page":"205","article-title":"Severe hemolytic disease of the newborn in a group B African\u2010American infant delivered by a group O mother","volume":"36","author":"Drabik\u2010clary K","year":"2006","journal-title":"Ann Card Anaesth"},{"issue":"2","key":"e_1_2_7_5_1","first-page":"99","article-title":"The incidence of spontaneous abortion in mothers with blood group O compared with other blood types","volume":"1","author":"Hassanzadeh\u2010nazarabadi M","year":"2012","journal-title":"Int J Mol Cell Med Spring"},{"issue":"3","key":"e_1_2_7_6_1","first-page":"202","article-title":"The frequency of ABO\u2010incompatible combinations in relation to maternal rhesus antibody values in Rh immunized women","volume":"17","author":"Vos GH","year":"1965","journal-title":"Am J Hum Genet"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.109.4.e53"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-2824.2008.00189.x"},{"key":"e_1_2_7_9_1","first-page":"119","volume-title":"Modern Blood Banking & Transfusion Practices","author":"Harmening D","year":"2013"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.2002.00407.x"},{"issue":"2","key":"e_1_2_7_11_1","first-page":"141","article-title":"A rare case of haemolytic disease of newborn with Bombay phenotype mother","volume":"7","author":"Shastry S","year":"2013","journal-title":"Asian J Trans Sci"},{"key":"e_1_2_7_12_1","first-page":"645","volume-title":"Technical Manual","author":"Josephson CD","year":"2011"},{"key":"e_1_2_7_13_1","first-page":"615","volume-title":"Nelson Textbook of Pediatrics","author":"Maheshwari A","year":"2012"},{"key":"e_1_2_7_14_1","first-page":"755","volume-title":"Rennie & Robertson's Textbook of Neonatology","author":"Murray NA","year":"2012"},{"key":"e_1_2_7_15_1","volume-title":"Technical Manual","author":"Roback JD","year":"2011"},{"issue":"4","key":"e_1_2_7_16_1","first-page":"388","article-title":"The prevalence of irregular erythrocyte antibodies among antenatal women in Delhi","volume":"9","author":"Pahuja S","year":"2011","journal-title":"Blood Transfus"},{"issue":"2","key":"e_1_2_7_17_1","first-page":"202","article-title":"Frequency and distribution of blood groups in the blood donors in western Ahmedabad\u2014a hospital based study","volume":"2","author":"Patel PA","year":"2012","journal-title":"Nat J Med Res"},{"key":"e_1_2_7_18_1","first-page":"22","article-title":"Prevalence of \u201cunexpected antibodies\u201d in the antenatal women attending the Government Maternity Hospital, Tirupati","volume":"4","author":"Suresh B","year":"2014","journal-title":"J Clin Sci Res"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118689943"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-6247.75998"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1988.tb03897.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1754.2005.00692.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41101258.x"},{"issue":"4","key":"e_1_2_7_24_1","first-page":"267","article-title":"Coombs\u2010negative immune hemolytic anemia","volume":"13","author":"B, G","year":"1976","journal-title":"Semin Hematol"},{"key":"e_1_2_7_25_1","first-page":"101","volume-title":"Modern Blood Banking & Transfusion Practices","author":"Green RE","year":"2013"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.5852.524"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/000992280404300108"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plabm.2015.10.001"},{"issue":"2","key":"e_1_2_7_29_1","first-page":"167","article-title":"Eluate testing following microscopically positive direct antiglobulin tests with anti\u2010IgG","volume":"37","author":"Richa E","year":"2007","journal-title":"Ann Clin Lab Sci"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2005.076794"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2009.01478.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02831348"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.31.3.283"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00444945"}],"container-title":["Transfusion Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tme.12718","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tme.12718","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tme.12718","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T06:21:58Z","timestamp":1693635718000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tme.12718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,24]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1111\/tme.12718"],"URL":"https:\/\/doi.org\/10.1111\/tme.12718","archive":["Portico"],"relation":{},"ISSN":["0958-7578","1365-3148"],"issn-type":[{"value":"0958-7578","type":"print"},{"value":"1365-3148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,24]]},"assertion":[{"value":"2018-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}