{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T03:05:56Z","timestamp":1776999956794,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T00:00:00Z","timestamp":1602201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to determine RHESUS D GENE (<jats:italic>RHD)<\/jats:italic>allelic variants among Croatian D\u2010negative blood donors and compare our results with respective data from other European countries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Altered or reduced D antigen expression can result in D variants, which can be mistyped and can lead to the alloimmunisation of the blood recipient.<jats:italic>RHD<\/jats:italic>genotyping can distinguish D variants: weak D, partial D and DEL, thus preventing alloimmunisation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material\/methods<\/jats:title><jats:p>A total of 6523 samples obtained from D\u2010negative Croatian donors were screened for the presence of<jats:italic>RHD<\/jats:italic>using the real\u2010time polymerase chain reaction (PCR) method. PCR\u2010SSP was performed for D variant genotyping by using commercial genotyping kits (Inno\u2010Train, Kronberg, Germany). Genomic DNA sequencing for all 10 exons of the<jats:italic>RHD<\/jats:italic>was performed when the genotyping kits failed to assign a D variant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>RHD<\/jats:italic>molecular screening revealed 23 (0.35%)<jats:italic>RHD<\/jats:italic>\u2010PCR positive samples, all C\/E positive, in decreasing frequency: 11 hybrid<jats:italic>RHD\u2010CE (2\u20109) D\u2010CE<\/jats:italic>variants, 4 weak partial D type 11 and 2 weak D type 2. Six samples remained unresolved and were sequenced. For 12 of 23 samples (excluding large hybrids), an adsorption\/elution of anti\u2010D serum was performed, confirming that all 12 were RhD+. The calculated frequency of clinically significant D alleles in RhD\u2010negative blood donors was 1:543 (0.18%) or 1:53 (1.89%) in C\/E blood donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Data on the significant frequency of D variants among serologically D\u2010negative blood donors in the north\u2010eastern region of Croatia could help in introducing<jats:italic>RHD<\/jats:italic>molecular screening of blood donors in a routine workflow.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12726","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T09:34:13Z","timestamp":1602236053000},"page":"43-47","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["D variants in the population of D\u2010negative blood donors in the north\u2010eastern region of Croatia"],"prefix":"10.1111","volume":"31","author":[{"given":"Hana","family":"Safic Stanic","sequence":"first","affiliation":[{"name":"Department of Molecular Diagnostics Croatian Institute of Transfusion Medicine Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6025-4649","authenticated-orcid":false,"given":"Vesna","family":"Dogic","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics Croatian Institute of Transfusion Medicine Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivona","family":"Herceg","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics Croatian Institute of Transfusion Medicine Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5754-9569","authenticated-orcid":false,"given":"Sandra","family":"Jagnjic","sequence":"additional","affiliation":[{"name":"Department of Immunohematology Croatian Institute of Transfusion Medicine Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9449-3154","authenticated-orcid":false,"given":"Jasna","family":"Bingulac\u2010Popovic","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics Croatian Institute of Transfusion Medicine Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0976-769X","authenticated-orcid":false,"given":"Ivana","family":"Babic","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics Croatian Institute of Transfusion Medicine Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4878-9173","authenticated-orcid":false,"given":"Ante","family":"Corusic","sequence":"additional","affiliation":[{"name":"Directory University Hospital Centre Zagreb Zagreb Croatia"},{"name":"School of Medicine University of Zagreb Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2778-8400","authenticated-orcid":false,"given":"Irena","family":"Jukic","sequence":"additional","affiliation":[{"name":"Medical Department Croatian Institute of Transfusion Medicine Zagreb Croatia"},{"name":"Faculty of Medicine Josip Juraj Strossmayer University of Osijek Osijek Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12275"},{"key":"e_1_2_7_3_1","first-page":"52","article-title":"The genetics of the Rhesus blood group system","volume":"5","author":"Flegel WA","year":"2007","journal-title":"Blood Transfus"},{"key":"e_1_2_7_4_1","unstructured":"International Society of Blood Transfusion. 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