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This study aimed to evaluate the prevalence of HEV\u2010RNA in blood donors in western India.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We screened 13\u2009050 blood donors for HEV using HEV\u2010RNA screening of 10 mini\u2010pools using RealStar HEV RT\u2010PCR Kit (95% limit of detection (LOD): 4.7\u00a0IU\/ml). Furthermore, all HEV\u2010RNA\u2010positive donors were investigated for the presence of IgM\/IgG antibody along with liver function tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 13\u2009050 blood donations, 7 (0.53%) were found to be HEV\u2010RNA positive, and the prevalence of HEV nucleic acid testing yield cases among blood donors was 1 in 1864. All seven HEV\u2010RNA\u2010positive samples were tested with anti\u2010HEV IgM and anti\u2010HEV IgG antibodies; this resulted in two (28.5%) positive anti\u2010HEV IgM and two (28.5%) positive anti\u2010HEV IgG antibodies. Hepatic activity was measured, with two of seven HEV\u2010RNA\u2010positive donors demonstrating abnormal serum glutamic oxaloacetic transaminase (SGOT) andserum glutamic pyruvic transaminase (SGPT). Two HEV\u2010RNA\u2010positive blood donors who had abnormal SGOT and SGPT were found to have a high HEV viral load. Furthermore, we were able to follow up two HEV\u2010RNA donors, and both were HEV\u2010RNA positive and had anti\u2010HEV IgM and anti\u2010HEV IgG antibodies; moreover, their liver function tests were also abnormal. One of the HEV\u2010RNA donors with high viral load did show hepatitis\u2010E\u2010like virus on electron microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our studies indicate that there is a significant risk of blood\u2010borne transmission of HEV. This finding may help to provide a direction towards the safety of blood transfusions in clinical settings in countries like India, which fall under the endemic category for HEV infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12760","type":"journal-article","created":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T02:01:35Z","timestamp":1616119295000},"page":"193-199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Risk of hepatitis\u2010E virus infections among blood donors in a regional blood transfusion centre in western India"],"prefix":"10.1111","volume":"31","author":[{"given":"Kanchan K.","family":"Mishra","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine Surat Raktadan Kendra and Research Centre  Surat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krima","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Transfusion 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