{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:04:14Z","timestamp":1747188254073,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,3]],"date-time":"2021-12-03T00:00:00Z","timestamp":1638489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the effects of cryopreservation in post\u2010thaw umbilical cord blood units for the survivability of Gram\u2010positive bacteria strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Microbial screening is required for all cord blood units (CBUs). Four gram\u2010positive contaminants were documented to survive cryopreservation poorly and isolation of other contaminants were reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty\u2010eight contaminated CBUs detected with either <jats:italic>Staphylococcus epidermidis<\/jats:italic>, <jats:italic>Corynebacterium species<\/jats:italic>, <jats:italic>Peptostreptococcus<\/jats:italic> or <jats:italic>Streptococcus species<\/jats:italic> before cryopreservation were used in this study. CBUs were processed, DMSO\u2010infused and microbial screened before cryopreservation. Post\u2010thaw microbial screening was achieved using 1 and 10\u00a0ml inoculants in BACTEC culture bottles. Positive bottles were subjected for microbial identification and results were compared with those from pre\u2010freeze.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A higher rate of microbial contamination was found using the 10\u00a0ml inoculant. Screening of 11 CBUs did not detect any contaminants while 30 CBUs screened detected more than one unknown contaminants and majority of contaminants were identified to be gram\u2010negative species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A higher inoculation volume used at post\u2010thaw for microbial screening improves contamination detection but leads to the loss of precious cord blood. Some contaminants did not survive cryopreservation or were not identified due to their low microbial levels. Contrasting contaminants found at post\u2010thaw suggest the improvements made in detection and identification of contaminants over the years.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12834","type":"journal-article","created":{"date-parts":[[2021,12,4]],"date-time":"2021-12-04T05:58:40Z","timestamp":1638597520000},"page":"82-87","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of cryopreservation on gram\u2010positive bacteria contaminants in umbilical cord blood"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8577-3202","authenticated-orcid":false,"given":"Kenny P. 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