{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T19:21:21Z","timestamp":1787340081161,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In low\u2010risk populations, variability in the sensitivity of current serological tests for Hepatitis C virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>) blood donor screening may lead to the presence of false\u2010positive results. This contributes to the unnecessary loss of blood donor samples as well as to difficulty in accurate donor counselling. The present study determined the optimal cut\u2010off value of a chemiluminescent immunoassay for identification of <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>\u2010reactive blood donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>In a retrospective cross\u2010sectional analysis of 193\u2009973 blood donations, 578 samples that were positive for <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> antibody in a chemiluminescent immunoassay and\/or <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> screening tests were identified. Blood from 379 of these positive samples was available for retesting by a second confirmatory <jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content> immunoassay followed by a receiver operating characteristic (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) curve analysis. Donors were also recalled for a new analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Only 71 (18.7%) blood samples remained HCV\u2010positive upon retesting, while 233 (61.5%) now tested negative and 75 (19.8%) yielding indeterminate results. A signal to cutoff ratio \u22654.32 was determined as the best differential threshold between a positive and negative result, increasing the positive predictive value from 27.3% to 66.7%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Using a higher threshold for an HCV\u2010positive blood sample enhances the chemiluminescent immunoassay screening test\u00b4s accuracy and helps to improve donor counselling and notification processes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12930","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T17:19:41Z","timestamp":1666027181000},"page":"159-164","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Improved detection of hepatitis C virus\u2010positive blood donors and determination of infection status"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0623-1999","authenticated-orcid":false,"given":"Anna S.","family":"Nishiya","sequence":"first","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Laboratory of Medical Investigation in Pathogenesis and targeted therapy in Oncoimmunohematology (LIM\u201031), Department of Hematology, Hospital das Cl\u00ednicas HCFMUSP, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8490-4634","authenticated-orcid":false,"given":"Cesar","family":"de Almeida\u2010Neto","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Disciplina de Ci\u00eancias M\u00e9dicas Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3502-3929","authenticated-orcid":false,"given":"Steven S.","family":"Witkin","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Tropical da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Department of Obstetrics and Gynecology Weill Cornell Medicine  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4190-0238","authenticated-orcid":false,"given":"Suzete C.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Laboratory of Medical Investigation in Pathogenesis and targeted therapy in Oncoimmunohematology (LIM\u201031), Department of Hematology, Hospital das Cl\u00ednicas HCFMUSP, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanci A.","family":"Salles","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1tima A. H.","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8533-8155","authenticated-orcid":false,"given":"Claudia Di Lorenzo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Universidade Federal de S\u00e3o Jo\u00e3o del\u2010Rei  S\u00e3o Jo\u00e3o del\u2010Rei Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0094-619X","authenticated-orcid":false,"given":"Vanderson","family":"Rocha","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Laboratory of Medical Investigation in Pathogenesis and targeted therapy in Oncoimmunohematology (LIM\u201031), Department of Hematology, Hospital das Cl\u00ednicas HCFMUSP, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Disciplina de Ci\u00eancias M\u00e9dicas Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP)  S\u00e3o Paulo Brazil"},{"name":"Churchill Hospital Oxford University  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3090-4575","authenticated-orcid":false,"given":"Alfredo","family":"Mendrone J\u00fanior","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Pr\u00f3\u2010Sangue Hemocentro de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Laboratory of Medical Investigation in Pathogenesis and targeted therapy in Oncoimmunohematology (LIM\u201031), Department of Hematology, Hospital das Cl\u00ednicas HCFMUSP, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"World Health Organization.Hepatitis C. 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