{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T13:08:27Z","timestamp":1725282507736},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T00:00:00Z","timestamp":1667001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to develop accurate and user\u2010friendly genetic assays to identify the inherited neutrophil antigen\u20102 (HNA\u20102) deficiency in humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>HNA\u20102 is one of the most important neutrophil antigens implicated in a number of human disorders. HNA\u20102 deficiency or HNA\u20102 null is a common phenotype observed in 3%\u20135% Americans. HNA\u20102 null individuals are at risk to produce isoantibodies (or alloantibodies) that play important roles in transfusion\u2010related acute lung injury, immune neutropenia, and bone marrow graft failure. We previously demonstrated that the <jats:italic>CD177<\/jats:italic> coding SNP 787A\u2009&gt;\u2009T (c.787A\u2009&gt;\u2009T) is the most important genetic determinant for HNA\u20102 deficiency. However, reliable genetic assays are not available for routine clinical laboratory application up to now.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>A novel polymerase chain reaction (PCR) strategy was used to determine genotypes of the <jats:italic>CD177<\/jats:italic> SNP c.787A\u2009&gt;\u2009T. In the simplified PCR assay, all allele specific primers and internal control primers were included in the same reaction, which ensures reliability of the assay. In addition, a novel high\u2010throughput nested TaqMan assay was developed to determine genotypes of c.787A\u2009&gt;\u2009T for large population genetic analysis of HNA\u20102 deficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>CD177<\/jats:italic> SNP c787A\u2009&gt;\u2009T genotypes of 396 subjects were 100% concordant among the single PCR reaction method, the nested TaqMan assay, and Sanger Sequencing analysis. Out of 396 subjects, all 18 donors with the <jats:italic>CD177<\/jats:italic> STP homozygous genotype were HNA\u20102 null.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The novel PCR\u2010based genotyping assay is accurate to identify HNA\u20102 deficient individuals and is suitable for clinical laboratories. In addition, the innovative high\u2010throughput nested TaqMan assay will be useful for large\u2010scale population screens and genetic studies of HNA\u20102 deficiency.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.12936","type":"journal-article","created":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T08:09:25Z","timestamp":1667030965000},"page":"68-74","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An accurate genetic assay to identify human neutrophil antigen 2 deficiency"],"prefix":"10.1111","volume":"33","author":[{"given":"Yunfang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences University of Minnesota  St. Paul Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randy M.","family":"Schuller","sequence":"additional","affiliation":[{"name":"American Red Cross, North Central Blood Services National Neutrophil Reference Laboratory  St. Paul Minnesota USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianming","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences University of Minnesota  St. Paul Minnesota 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