{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:04:40Z","timestamp":1747188280672,"version":"3.40.5"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T00:00:00Z","timestamp":1731715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>At the regional transfusion service in the Region of Southern Denmark, serological investigations are primarily carried out using column agglutination techniques. This case study examines an unusual instance of reagent interference in pretransfusion testing using column agglutination at the Hospital of Southern Denmark, Aabenraa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Case Presentation<\/jats:title><jats:p>A 72\u2010year\u2010old male patient presented for pretransfusion testing prior to hernia surgery. He typed as O RhD negative without discrepancies, but the antibody screen showed weakly positive reactions. Routine investigations showed discrepancies, leading to further investigations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Various serological tests were performed using in\u2010house and commercial red test cells suspended in different suspension media and with different column agglutination cards and cassettes. Further investigations included washing of test cells, testing alternative saline solutions, varying incubation temperatures, testing without antihuman\u2010globulin, and applying proteolytic enzymes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Reactivity was present with red cells suspended in ID\u2010CellStab (BioRad) but not in Red Cell Diluent (Quidel\u2010Ortho). Reactivity was abolished by using trypsin\u2010treated cells, indicating either the presence of an antibody reacting with\u2014or unspecific agglutination depending on\u2014a trypsin\u2010sensitive protein, in both cases enhanced by ID\u2010CellStab.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This case highlights the importance of recognising reagent\u2010dependent reactivity in serological testing. Adjustments to the suspension media resolved the incompatibility. Immunohematology laboratories should consider potential reagent interference when unexpected agglutination occurs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.13113","type":"journal-article","created":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T10:54:43Z","timestamp":1731754483000},"page":"192-196","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An unusual case of reagent interference in transfusion medicine workup: Pan reactive indirect antiglobulin test caused by commercial saline solution"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9087-5977","authenticated-orcid":false,"given":"Martin T\u00f8ffner","family":"Pedersen","sequence":"first","affiliation":[{"name":"Department of Blood tests, Biochemistry and Immunology University Hospital of Southern Denmark  Aabenraa Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helle","family":"Bach\u2010Hansen","sequence":"additional","affiliation":[{"name":"Department of Blood tests, Biochemistry and Immunology University Hospital of Southern Denmark  Aabenraa Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina Fruerlund","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark H.","family":"Yazer","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Odense University Hospital  Odense Denmark"},{"name":"Department of Pathology University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4426-5054","authenticated-orcid":false,"given":"Ulrik","family":"Sprog\u00f8e","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,16]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Ortho\u2010Clinical Diagnostics.Anti\u2010Human Globulin Anti\u2010IgG (Rabbit) (Green) Ortho BioVue\u00ae System (IgG Cassette) INSTRUCTIONS FOR USE [Internet].Technical Documents (quidelortho.com"},{"key":"e_1_2_9_3_1","unstructured":"Coombs Anti\u2010IgG | Bio\u2010Rad [Internet]. Accessed April 5. 2024.Coombs Anti\u2010IgG | Bio\u2010Rad"},{"key":"e_1_2_9_4_1","unstructured":"ID\u2010CellStab | Bio\u2010Rad [Internet]. Accessed April 5 2024.ID\u2010CellStab | Bio\u2010Rad"},{"key":"e_1_2_9_5_1","unstructured":"Technical Documents RCD 0 8% [Internet]. Accessed April 5 2024.QuidelOrtho_Red_Cell_Diluent"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-052"},{"key":"e_1_2_9_7_1","unstructured":"Technical Documents B\u2010panel [Internet]. Accessed April 5 2024.QuidelOrtho_Resolve_Panel_B"},{"key":"e_1_2_9_8_1","unstructured":"ID\u2010Diluent 2 | Bio\u2010Rad [Internet]. Accessed April 5 2024.ID\u2010Diluent 2 | Bio\u2010Rad"},{"key":"e_1_2_9_9_1","first-page":"1038","volume-title":"Technical Manual","author":"Roback JD","year":"2011"},{"issue":"6","key":"e_1_2_9_10_1","first-page":"209","article-title":"Problems in pre\u2010transfusion tests related to drugs and chemicals","volume":"42","author":"Garratty G","year":"1976","journal-title":"Am J Med Technol"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/ajts.AJTS_100_17"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/ajts.AJTS_99_20"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03327.x"},{"key":"e_1_2_9_14_1","first-page":"238","volume-title":"The Blood Group Antigen: Factsbook","author":"Reid ME","year":"2004"}],"container-title":["Transfusion Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tme.13113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T16:07:56Z","timestamp":1744733276000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tme.13113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,16]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1111\/tme.13113"],"URL":"https:\/\/doi.org\/10.1111\/tme.13113","archive":["Portico"],"relation":{},"ISSN":["0958-7578","1365-3148"],"issn-type":[{"type":"print","value":"0958-7578"},{"type":"electronic","value":"1365-3148"}],"subject":[],"published":{"date-parts":[[2024,11,16]]},"assertion":[{"value":"2024-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}