{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T17:40:06Z","timestamp":1777052406037,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Granulocyte transfusions represent a therapeutic option for severely neutropenic patients with bacterial or fungal infections that are otherwise unresponsive to conventional therapy. Prior clinical studies suggest that patients receiving higher granulocyte doses achieve superior outcomes. Consequently, suboptimal donor stimulation and collection leading to lower granulocyte doses likely correlate with worse clinical outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design<\/jats:title><jats:p>A retrospective analysis was conducted on mobilisation data from 312 granulocyte collections from healthy donors between January 2020 and May 2023. This study was performed in a single blood donor center exclusively supporting a comprehensive cancer center. Donors underwent stimulation with 480\u2009mcg of filgrastim (granulocyte colony stimulating factor [G\u2010CSF]) subcutaneously and 8\u2009mg of dexamethasone orally administered 12 to 14\u2009h before collection. The correlation between donor characteristics (age, gender, body weight (BW), body mass index (BMI), baseline haemoglobin (Hgb), and platelet (PLT) counts) and mobilisation efficiency (\u0394 WBC, defined as post\u2010mobilisation WBC count\u2014baseline WBC count) was examined to identify factors associated with enhanced mobilisation efficiency. Additionally, the impact of multiple donations on \u0394 WBC in repeat donors was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The median donor age was 43\u2009years (range 18\u201381), with 224 male and 88 female donors. Female donors exhibited significantly higher baseline PLT counts and post\u2010mobilisation WBC counts. However, donor gender did not significantly affect \u0394 WBC. A negative correlation was observed between \u0394 WBC and age (<jats:italic>r<\/jats:italic>\u2009=\u2009\u22120.235, <jats:italic>p<\/jats:italic>\u2009=\u20090.001), with older donors (61\u201381\u2009years) exhibiting significantly lower mobilisation efficiency. BW and BMI differences had no significant effect on \u0394 WBC. A positive correlation was identified between baseline PLT count and \u0394 WBC (<jats:italic>r<\/jats:italic>\u2009=\u20090.140, <jats:italic>p<\/jats:italic>\u2009=\u20090.014), with females having significantly higher baseline PLT counts (<jats:italic>p<\/jats:italic>\u2009=\u20090.0004). No correlation was found between \u0394 WBC and baseline Hgb (<jats:italic>r<\/jats:italic>\u2009=\u20090.004, <jats:italic>p<\/jats:italic>\u2009=\u20090.477). Repeat donors showed no statistically significant change in \u0394 WBC with subsequent donations, with a mean interval of 136.5\u2009days between collections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mobilisation efficiency was not impacted by donor BW or BMI suggesting that BW\u2010based G\u2010CSF stimulation is not essential for optimising WBC mobilisation. Rather, a fixed single dose of 480 mcg of G\u2010CSF and 8\u2009mg of dexamethasone was sufficient to mobilise donors, thus reducing the procedural costs and the potential risks for medication\u2010related side effects. The positive correlation found between baseline PLT count and \u0394 WBC suggests that PLT count could be used as a potential predictor of mobilisation efficiency. Mobilisation response in up to four collections in repeat granulocytes donors was not affected in subsequent donations. However, sample size is a limitation, and more data is needed for a meaningful conclusion of whether frequent granulocyte donations are safe and effective.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.13115","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T10:44:22Z","timestamp":1732704262000},"page":"177-183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Factors influencing white blood cell mobilisation in healthy granulocyte donors"],"prefix":"10.1111","volume":"35","author":[{"given":"Roman","family":"Gonta","sequence":"first","affiliation":[{"name":"Division of Pathology and Laboratory Medicine The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Division of Pathology and Laboratory Medicine Moffitt Cancer Center  Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason M.","family":"Schenkel","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"Klein","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Martinez","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine The University of Texas MD Anderson Cancer Center  Houston Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayrin Correa","family":"Medina","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine The University of Texas MD Anderson Cancer Center  Houston Texas USA"},{"name":"Division of Pathology and Laboratory Medicine Moffitt Cancer Center  Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,27]]},"reference":[{"issue":"15","key":"e_1_2_10_2_1","first-page":"15","article-title":"Granulocyte transfusions as a therapy and a prophylaxis of infections in neutropenic patients","volume":"1979","author":"Klastersky J","year":"1965","journal-title":"Eur J Cancer"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423\u20100410.2008.01159.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198105143042001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000460849"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003\u20104819\u201096\u20102\u2010149"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102015\u201005\u2010645986"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2014.12.006"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20710"},{"issue":"1","key":"e_1_2_10_10_1","first-page":"71","article-title":"Combined treatment with amphotericin\u2010B and granulocyte transfusion from G\u2010CSF\u2010stimulated donors in an aplastic patient with invasive aspergillosis undergoing bone marrow transplantation","volume":"82","author":"Catalano L","year":"1997","journal-title":"Haematologica"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/16.4.528"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537\u20102995.2007.01444.x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.2008.06996.x"},{"key":"e_1_2_10_14_1","unstructured":"American Association of Blood Banks.Committee on Standards. 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