{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T12:16:34Z","timestamp":1767183394103,"version":"3.43.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T00:00:00Z","timestamp":1738108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>High\u2010quality ABO antibody titre testing is required for ABO\u2010incompatible haematopoietic stem cell transplantation and kidney transplantation. To assess the feasibility of automated ABO titration as an alternative to manual and semi\u2010automatic titration during the peri\u2010transplant period, a comparative study was conducted internally in a transfusion medicine laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>This study was performed in two stages. Firstly, the differences in anti\u2010A\/B detecting using conventional tube test (CTT) and manual column agglutination technology (CAT) were compared in group O pregnant women. Then, manual and automated CAT were applied to simultaneously detect ABO antibody levels in non\u2010AB\u2010group adult haematological oncology patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the first stage, four groups of 192 comparable results were generated from 48 subjects, which showed a high correlation between CTT and manual CAT (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). However, the detection sensitivity of the latter was at least 1 fold higher dilution than that of the former. Fifty\u2010six patients were tested in the second stage by simultaneous manual and automated CAT, and the paired titers differed within an acceptable range (\u00b11 fold dilution). However, the concordance was slightly higher in group IgM (85.4%) than in group IgG (80.5%). Blood group specificity of ABO titers was also observed in this population, and no association between titers and other factors was found.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Automated CAT standardises ABO titre testing and saves labor costs, although manual review of weak positive reactions is still necessary. Due to the blood group specificity of ABO antibodies, special attention should be paid to the quality control and clinical relevance of titre testing for group O recipients in ABO\u2010mismatched transplantation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tme.13127","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T00:25:03Z","timestamp":1738196703000},"page":"258-265","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Practice of <scp>ABO<\/scp> antibody titration in a transplant center: From tube method to manual gel column testing to automated column agglutination technology"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3021-8436","authenticated-orcid":false,"given":"Jun\u2010Bo","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Blood Transfusion The Second Affiliated Hospital of Anhui Medical University  Hefei 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