{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T13:41:22Z","timestamp":1770558082345,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/50093\u20108"],"award-info":[{"award-number":["2014\/50093\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Zika virus (ZIKV) is primarily transmitted through the bite of the\n                      <jats:italic>Aedes aegypti<\/jats:italic>\n                      mosquito, though transmission via blood transfusion has also been documented. During the 2015 ZIKV epidemic in Brazil, severe complications were observed in pregnant women, leading to fetal microcephaly. This study evaluated the persistence of ZIKV in blood donated by healthy individuals during the post\u2010epidemic period from 2016 to 2020.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood donor samples from 109\u2009296 individuals were screened for ZIKV RNA using nucleic acids extracted from plasma pools (six donors per pool). These samples had previously undergone routine nucleic acid testing (NAT) for HBV, HCV and HIV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Viral RNA (Ribonucleic Acid) was detected in a donor sample from the city of Santos in May 2016, resulting in a prevalence of 0.0009%. The positive donor was confirmed through viral sequencing using the Sanger method. Sequencing and phylogenetic analysis of an envelope gene amplicon revealed that the Zika virus RNA detected belonged to the Asian clade. This Asian lineage strain emerged in Brazil, Fortaleza, in 2015, isolated in northeastern Brazil in 2015, an area where most cases of microcephaly associated with ZIKV have been reported. A follow\u2010up sample collected 1\u2009month after donation showed seroconversion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The detection of ZIKV RNA by NAT in a donated blood sample demonstrates that, although extremely rare, the virus is still present. Periodic active surveillance of blood donations for viruses associated with past outbreaks may help identify an incipient resurgence before it develops into a new epidemic.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tme.70030","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T23:03:31Z","timestamp":1760655811000},"page":"59-65","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zika virus surveillance post\u2010epidemic in blood donors from S\u00e3o Paulo, Brazil 2016\u20132020"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4190-0238","authenticated-orcid":false,"given":"Suzete Cleusa","family":"Ferreira","sequence":"first","affiliation":[{"name":"Departamento de Seguran\u00e7a Transfusional Funda\u00e7\u00e3o Pro Sangue Hemocentro de S\u00e3o Paulo, Divis\u00e3o de Pesquisa  S\u00e3o Paulo SP Brazil"},{"name":"Laboratory of Medical Investigation in Pathogenesis and targeted therapy in Onco\u2010Immuno\u2010Hematology (LIM\u201031), Department of Hematology, Hospital das Cl\u00ednicas HCFMUSP, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3557-2717","authenticated-orcid":false,"given":"Jos\u00e9 Eduardo","family":"Levi","sequence":"additional","affiliation":[{"name":"Departamento de Virologia Universidade de S\u00e3o Paulo \u2010Instituto de Medicina Tropical  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Shoko","family":"Nishiya","sequence":"additional","affiliation":[{"name":"Departamento de Seguran\u00e7a Transfusional Funda\u00e7\u00e3o Pro Sangue Hemocentro de S\u00e3o Paulo, Divis\u00e3o de Pesquisa  S\u00e3o Paulo SP Brazil"},{"name":"Laboratory of Medical Investigation in Pathogenesis and targeted therapy in Onco\u2010Immuno\u2010Hematology (LIM\u201031), Department of Hematology, Hospital das Cl\u00ednicas HCFMUSP, Faculdade de Medicina Universidade de S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8490-4634","authenticated-orcid":false,"given":"Cesar","family":"de Almeida\u2010Neto","sequence":"additional","affiliation":[{"name":"Departamento de Seguran\u00e7a Transfusional Funda\u00e7\u00e3o Pro Sangue Hemocentro de S\u00e3o Paulo, Divis\u00e3o de Pesquisa  S\u00e3o Paulo SP Brazil"},{"name":"Faculdade de Medicina da Universidade de S\u00e3o Paulo Disciplina de Ci\u00eancias M\u00e9dicas  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerenice Esdras","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Hematologia e Bioqu\u00edmica Instituto Adolf Lutz, Divis\u00e3o de Patologia  S\u00e3o Paulo SP 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