{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T09:35:46Z","timestamp":1792056946409,"version":"4.4.0"},"reference-count":61,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Thrombocytopenia arises from heterogeneous inherited and acquired disorders, and identifying the underlying platelet clearance mechanisms remains challenging. Platelet desialylation, characterised by loss of sialic acid and consequent exposure of terminal \u03b2\u2010galactose residues recognised by the Ashwell\u2013Morell receptor, represents an alternative pathway of hepatic platelet clearance. This study aimed to validate a modified platelet desialylation test (PDT) and assess its applicability in multitransfused, alloimmunized, and hereditary thrombocytopenia patients as a functional in vitro assay to detect plasma\u2010induced platelet desialylation and explore its potential as a complementary biomarker of platelet clearance mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>PDT performance was evaluated using plasma from 20 healthy donor pools, 10 antibody\u2010positive refractory patients, and 15 individuals with hereditary thrombocytopenias by FITC\u2013RCA\u2010I flow cytometry. Analytical validation included assessment of reproducibility, incubation time, lectin concentration, and ratio\u2010based result interpretation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      \u03b2\u2010Lactose showed significantly lower expression mean fluorescence intensity (MFI) than both untreated reference control and neuraminidase\u2010treated conditions (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.00001), whereas patient plasma exhibited higher fluorescence than the reference control (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). A 1\u2009h incubation using 0.3\u2009\u03bcL per reaction RCA\u2010I\u2010FITC stock solution (5\u2009mg active conjugate\/mL) provided stable and reproducible discrimination. In inherited thrombocytopenias, 66.7% of patients were PDT\u2010positive despite HLA\/HPA antibodies being detected in only 13.3%. PDT\u2010positive samples showed increased desialylation\u2010associated MFI (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002) with a strong correlation between MFI and ratio (\n                      <jats:italic>\u03c1<\/jats:italic>\n                      \u2009=\u20090.877). The assay required approximately 4\u2009h and cost US$3.62 per test.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The PDT is a practical, reproducible, and cost\u2010effective assay capable of detecting physiologic, enzymatic, and immune\u2010mediated platelet desialylation, providing a complementary biomarker for investigating thrombocytopenic disorders.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tme.70096","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T11:37:16Z","timestamp":1781696236000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Functional validation of a modified platelet desialylation test for immune and hereditary thrombocytopenias"],"prefix":"10.1111","author":[{"given":"Karen Nogueira 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