{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T07:14:49Z","timestamp":1783322089612,"version":"3.54.6"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002784","name":"Canada Excellence Research Chairs, Government of Canada","doi-asserted-by":"publisher","award":["CERC\u20102022\u201000072"],"award-info":[{"award-number":["CERC\u20102022\u201000072"]}],"id":[{"id":"10.13039\/501100002784","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>A novel meniscus\u2010based analysis provides a rapid method to identify analytical errors related to unrecognised pipetting errors.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The indirect antihuman globulin test (IAT) is part of pretransfusion testing, enabling detection of erythrocyte alloantibodies. Failure to detect such antibodies may result in transfusion complications. In column agglutination technique (CAT)\u2010based IAT, correct separation of the different liquids by an air gap during incubation is essential. Penetration of this air gap due to pipetting errors can cause neutralisation of AHG, resulting in false\u2010negative or weak test results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      We investigated the fluid dynamics of CAT\u2010based IAT and developed a simple image\u2010based quality control approach to detect air gap penetration. Using routinely generated analyser images, the meniscus contact angle was quantified with the software\n                      <jats:italic>ImageJ<\/jats:italic>\n                      in 132 simulated crossmatch tests.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Receiver operating characteristic analysis demonstrated high discriminatory performance (AUC 0.935). Additional experiments using bovine serum albumin solutions confirmed that increased protein concentration significantly reduces meniscus contact angle, supporting the proposed mechanism of error prediction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>While further validation across platforms and laboratories is required, this approach has the potential to enhance test reliability, support troubleshooting, and improve patient safety in routine IAT CAT workflows.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/tme.70103","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T06:33:06Z","timestamp":1783319586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simple image analysis for reliability control of column agglutination test in pretransfusion testing"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9087-5977","authenticated-orcid":false,"given":"Martin T\u00f6ffner","family":"Pedersen","sequence":"first","affiliation":[{"name":"Department of Blood Tests, Biochemistry and Immunology University Hospital of Southern Denmark  Aabenraa Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tharisa","family":"Rajaguru","sequence":"additional","affiliation":[{"name":"Department of Blood Tests, Biochemistry and Immunology University Hospital of Southern Denmark  Aabenraa Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6510-8372","authenticated-orcid":false,"given":"Albert","family":"Ashong","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramis","family":"Arbi","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8767-9623","authenticated-orcid":false,"given":"Niko","family":"Hildebrandt","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics McMaster University  Hamilton Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thor","family":"Skovsted","sequence":"additional","affiliation":[{"name":"Department of Blood Tests, Biochemistry and Immunology University Hospital of Southern Denmark  Aabenraa Denmark"},{"name":"Department of Regional Health Research University of Southern Denmark  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000277"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1677581"},{"issue":"3","key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.29074\/ascls.24.3.161","article-title":"Variation in pipetting may lead to the decreased detection of antibodies in manual gel testing","volume":"24","author":"Bobryk S","year":"2011","journal-title":"Am Soc Clin Lab Sci"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14645"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537\u20102995.2009.02464.x"},{"key":"e_1_2_11_7_1","unstructured":"DSKI H committee.Members of Haemovigilance committee DSKI.2025https:\/\/dski.dk\/wp-content\/uploads\/2025\/12\/haemovigilance-22-24-final.pdf"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/tme.12002"},{"key":"e_1_2_11_9_1","unstructured":"DSKI.tms\u20105.6\u2010final.pdf. accessed December 30 2025.https:\/\/dski.dk\/wp-content\/uploads\/2024\/08\/tms-5.6-final.pdf"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537\u20102995.1993.33893342744.x"},{"key":"e_1_2_11_11_1","unstructured":"Ortho\u2010Clinical Diagnostics.Anti\u2010Human Globulin Anti\u2010IgG (Rabbit) (Green) Ortho BioVue\u00ae System (IgG Cassette) INSTRUCTIONS FOR USE. 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