{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:22:26Z","timestamp":1759638146303},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,4]],"date-time":"2013-01-04T00:00:00Z","timestamp":1357257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2013,4]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>To evaluate the accuracy of a peptide, corresponding to the variant surface glycoprotein (VSG<\/jats:styled-content>) LiTat 1.5 amino acid (AA<\/jats:styled-content>) sequence 268\u2013281 and identified through alignment of monoclonal antibody selected mimotopes, for diagnosis of T<\/jats:styled-content>rypanosoma brucei gambiense<\/jats:italic> sleeping sickness.<\/jats:p><\/jats:sec>Methods<\/jats:title>A synthetic biotinylated peptide (peptide 1.5\/268\u2013281), native VSG<\/jats:styled-content> LiTat 1.3 and VSG<\/jats:styled-content> LiTat 1.5 were tested in an indirect ELISA<\/jats:styled-content> with 102 sera from patients with HAT<\/jats:styled-content> and 102 endemic HAT<\/jats:styled-content>\u2010negative controls.<\/jats:p><\/jats:sec>Results<\/jats:title>The area under the curve (AUC<\/jats:styled-content>) of peptide 1.5\/268\u2013281 was 0.954 (95% confidence interval 0.918\u20130.980), indicating diagnostic potential. The areas under the curve of VSG<\/jats:styled-content> LiTat 1.3 and LiTat 1.5 were 1.000 (0.982\u20131.000) and 0.997 (0.973\u20131.000), respectively, and significantly higher than the AUC<\/jats:styled-content> of peptide 1.5\/268\u2013281. On a model of VSG<\/jats:styled-content> LiTat 1.5, peptide 1.5\/268\u2013281 was mapped near the top of the VSG<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>A biotinylated peptide corresponding to AA<\/jats:styled-content> 268\u2013281 of VSG<\/jats:styled-content> LiTat 1.5 may replace the native VSG<\/jats:styled-content> in serodiagnostic tests, but the diagnostic accuracy is lower than for the full\u2010length native VSG<\/jats:styled-content> LiTat 1.3 and VSG<\/jats:styled-content> LiTat 1.5.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12058","type":"journal-article","created":{"date-parts":[[2013,1,4]],"date-time":"2013-01-04T16:22:03Z","timestamp":1357316523000},"page":"461-465","source":"Crossref","is-referenced-by-count":9,"title":["A L<\/scp>iT<\/scp>at 1.5 variant surface glycoprotein\u2010derived peptide with diagnostic potential for T<\/scp>rypanosoma brucei gambiense<\/i>"],"prefix":"10.1111","volume":"18","author":[{"given":"Liesbeth","family":"Van Nieuwenhove","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"B\u00fcscher","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Balharbi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Institute of Tropical Medicine Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Humbert","sequence":"additional","affiliation":[{"name":"Dana\u2010Farber Cancer Institute Boston MA USA"},{"name":"Harvard Medical School Boston MA 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