{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T19:15:56Z","timestamp":1784747756342,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,27]],"date-time":"2013-04-27T00:00:00Z","timestamp":1367020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify the <jats:italic>Anopheles culicifacies<\/jats:italic> sibling species complex and study their vectorial role in malaria endemic regions of Odisha.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mosquitoes were collected from 6 malaria endemic districts using standard entomological collection methods. <jats:italic>An.\u00a0culicifacies<\/jats:italic> sibling species were identified by multiplex polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) using cytochrome oxidase subunit <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">COII<\/jats:styled-content>) region of mitochondrial <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. <jats:italic>Plasmodium falciparum<\/jats:italic> (Pf) sporozoite rate and human blood fed percentage (<jats:styled-content style=\"fixed-case\">HBF<\/jats:styled-content>) were estimated by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> using Pf\u2010 and human\u2010specific primers. Sequencing and phylogenetic analysis were performed to confirm the type of sibling species of <jats:italic>An.\u00a0culicifacies<\/jats:italic> found in Odisha.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> detected <jats:italic>An.\u00a0culicifacies<\/jats:italic> sibling species A, B, C, D and E in the malaria endemic regions of Odisha. <jats:italic>An.\u00a0culicifacies<\/jats:italic> E was detected for the first time in Odisha, which was further confirmed by molecular phylogenetics. Highest sporozoite rate and <jats:styled-content style=\"fixed-case\">HBF<\/jats:styled-content> percentage were observed in <jats:italic>An.\u00a0culicifacies<\/jats:italic> E in comparison with other sibling species. <jats:italic>An.\u00a0culicifacies<\/jats:italic> E collected from Nawarangapur, Nuapara and Keonjhar district showed high <jats:styled-content style=\"fixed-case\">HBF<\/jats:styled-content> percentage and sporozoite rates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>An.\u00a0culicifacies<\/jats:italic> B was the most abundant species, followed by <jats:italic>An.\u00a0culicifacies<\/jats:italic> C and E. High sporozoite rate and <jats:styled-content style=\"fixed-case\">HBF<\/jats:styled-content> of <jats:italic>An.\u00a0culicifacies<\/jats:italic> E indicated that it plays an important role in malaria transmission in Odisha. Appropriate control measures against <jats:italic>An.\u00a0culicifacies<\/jats:italic> E at an early stage are needed to prevent further malaria transmission in Odisha.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12112","type":"journal-article","created":{"date-parts":[[2013,4,27]],"date-time":"2013-04-27T08:18:49Z","timestamp":1367050729000},"page":"810-821","source":"Crossref","is-referenced-by-count":21,"title":["<i>Anopheles culicifacies<\/i> sibling species in Odisha, eastern India: First appearance of <i>Anopheles culicifacies<\/i> E and its vectorial role in malaria transmission"],"prefix":"10.1111","volume":"18","author":[{"given":"Mumani","family":"Das","sequence":"first","affiliation":[{"name":"Department of Medical Entomology Regional Medical Research Centre Chandrasekharpur Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biswadeep","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology Regional Medical Research Centre Chandrasekharpur Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparna P.","family":"Patra","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology Regional Medical Research Centre Chandrasekharpur Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hare K.","family":"Tripathy","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology Regional Medical Research Centre Chandrasekharpur Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namita","family":"Mohapatra","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology Regional Medical Research Centre Chandrasekharpur Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santanu K.","family":"Kar","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology Regional Medical Research Centre Chandrasekharpur Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupenangshu K.","family":"Hazra","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology Regional Medical Research Centre Chandrasekharpur Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2008.09.017"},{"key":"e_1_2_6_3_1","volume-title":"The Fauna of British India. 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