{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T20:08:41Z","timestamp":1770235721027,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,6,3]],"date-time":"2013-06-03T00:00:00Z","timestamp":1370217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>There are little data available on the pathology caused by the sibling species <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>nisakis simplex<\/jats:italic> s.s. and <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>nisakis pegreffii<\/jats:italic>. The differences shown in their ability to penetrate the muscle of fish may also be manifested in humans. The purpose of this study is to confirm possible differences in pathogenicity between <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0simplex<\/jats:italic> s.s. and <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0pegreffii<\/jats:italic> using an experimental model which simulates infection in humans.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Female <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>istar rats were infected with 190 <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>nisakis<\/jats:italic> type I <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>3 larvae from the <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>berian coastline. After the animal was sacrificed, these <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>3 larvae were then recovered and identified via <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> of the <jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content>1\u20105.8<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">ITS<\/jats:styled-content>2. A logistic regression analysis was performed searching for association between experimental pathogenic potential and species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The distribution of <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0simplex<\/jats:italic> s.s. and <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0pegreffii<\/jats:italic> between <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>tlantic and <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>editerranean waters of the <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>berian <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>eninsula showed statistically significant differences (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001) which were not observed in the hybrid genotypes (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.3). 21.6% showed pathogenic potential, interpreted as the capacity of the larvae to cause lesions, stick to the gastrointestinal wall or penetrate it. The species variable showed association with the pathogenic role of the larva (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.008). Taking <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0simplex<\/jats:italic> s.s. as our reference, the <jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content> for <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0pegreffii<\/jats:italic> is 0.351 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.028).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Despite this difference, <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0pegreffii<\/jats:italic> is also capable of causing anisakiasis, being responsible for 14.3% of the penetrations of the gastric mucosa found in rats, which justifies both species being considered aetiologic agents of this parasitic disorder.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12131","type":"journal-article","created":{"date-parts":[[2013,6,3]],"date-time":"2013-06-03T10:42:15Z","timestamp":1370256135000},"page":"979-984","source":"Crossref","is-referenced-by-count":46,"title":["Experimental comparison of pathogenic potential of two sibling species <i><scp>A<\/scp>nisakis simplex<\/i> s.s. and <i><scp>A<\/scp>nisakis pegreffii<\/i> in <scp>W<\/scp>istar rat"],"prefix":"10.1111","volume":"18","author":[{"given":"Mar\u00eda","family":"del Carmen Romero","sequence":"first","affiliation":[{"name":"Parasitology Department Faculty of Pharmacy University of Granada Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adela","family":"Valero","sequence":"additional","affiliation":[{"name":"Parasitology Department Faculty of Pharmacy University of Granada Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Concepci\u00f3n","family":"Navarro\u2010Moll","sequence":"additional","affiliation":[{"name":"Pharmacology Department Faculty of Pharmacy University of Granada Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaquina","family":"Mart\u00edn\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Parasitology Department Faculty of Pharmacy University of Granada Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2011.07.026"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2011.1076"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00012-07"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/00364827.1961.10410245"},{"key":"e_1_2_6_6_1","first-page":"463","article-title":"Anisakiasis digestiva: cl\u00ednica y diagn\u00f3stico seg\u00fan localizaci\u00f3n","volume":"94","author":"Cast\u00e1n B","year":"2002","journal-title":"Revista Espa\u00f1ola de Enfermedades Digestivas"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2009-5"},{"key":"e_1_2_6_8_1","first-page":"591","article-title":"Diagnosis of a case of gastric anisakidosis by PCR\u2010based restriction fragment length polymorphism analysis","volume":"41","author":"D'Amelio S","year":"1999","journal-title":"Parassitologia"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00178-2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(00)90194-5"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2005.11.007"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0325"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2718-4"},{"key":"e_1_2_6_14_1","first-page":"43","volume-title":"Progress in Clinical Parasitology","author":"Ishikura H","year":"1992"},{"key":"e_1_2_6_15_1","first-page":"437","article-title":"Multiple acute parasitization by Anisakis simplex","volume":"20","author":"Jurado\u2010Palomo J","year":"2010","journal-title":"Journal of Investigational Allergology and Clinical Immunology"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00785326.1993.10429897"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.02.005"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)00202-9"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X07754718"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-11-82"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.09.008"},{"key":"e_1_2_6_22_1","volume-title":"Sobotta, Atlas of Anatomy, Volume 2: Internal Organs","author":"Paulsen F","year":"2011"},{"key":"e_1_2_6_23_1","volume-title":"Algunos aspectos de la epidemiolog\u00eda y prevenci\u00f3n de la anisakiasis. 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