{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:21:52Z","timestamp":1787016112493,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2013,10]]},"abstract":"Abstract<\/jats:title>\n \n Objective<\/jats:title>\n \n To evaluate the potential involvement of anti\u2010\n \n T<\/jats:styled-content>\n rypanosoma cruzi\n <\/jats:italic>\n and cardiac protein antibody (\n I<\/jats:styled-content>\n gG total and isotypes) production and their possible association with different clinical forms of human chronic\n C<\/jats:styled-content>\n hagas disease.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n IgG total and isotypes were measured by\n ELISA<\/jats:styled-content>\n , using epimastigote and trypomastigote forms of\n \n T<\/jats:styled-content>\n .\u00a0cruzi\n <\/jats:italic>\n as antigens and human cardiac proteins (myosin and troponin\n T<\/jats:styled-content>\n ) in sera of patients with indeterminate (\n IND<\/jats:styled-content>\n ,\n n<\/jats:italic>\n \u00a0=\u00a072), cardiac (\n CARD<\/jats:styled-content>\n ,\n n<\/jats:italic>\n \u00a0=\u00a047) and digestive\/cardiodigestive (\n DIG<\/jats:styled-content>\n \/\n CARD<\/jats:styled-content>\n \u2010\n DIG<\/jats:styled-content>\n ,\n n<\/jats:italic>\n \u00a0=\u00a012) clinical forms of the disease. Samples from uninfected health individuals (\n CONT<\/jats:styled-content>\n ,\n n<\/jats:italic>\n \u00a0=\u00a030) and patients with ischaemic cardiomyopathy (\n ISCH<\/jats:styled-content>\n ,\n n<\/jats:italic>\n \u00a0=\u00a015) were used as controls. Autoantibody levels were correlated with parameters of cardiac function obtained by electrocardiographic, radiographic and echocardiographic examinations.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Fifty five per cent of patients were classified as\n IND<\/jats:styled-content>\n , 35.9% as\n CARD<\/jats:styled-content>\n and 9.1% as\n DIG<\/jats:styled-content>\n \/\n CARD<\/jats:styled-content>\n \u2010\n DIG<\/jats:styled-content>\n . Greater total\n I<\/jats:styled-content>\n gG production was observed in\n IND<\/jats:styled-content>\n ,\n CARD<\/jats:styled-content>\n and\n DIG<\/jats:styled-content>\n \/\n CARD<\/jats:styled-content>\n \u2010\n DIG<\/jats:styled-content>\n chagasic patients than in\n CONT<\/jats:styled-content>\n and\n ISCH<\/jats:styled-content>\n , using trypomastigote, epimastigote and cardiac antigens. Moreover, patients with\n CARD<\/jats:styled-content>\n and\n DIG<\/jats:styled-content>\n \/\n CARD<\/jats:styled-content>\n \u2010\n DIG<\/jats:styled-content>\n presented greater total\n I<\/jats:styled-content>\n gG production (trypomastigote and epimastigote antigen) than\n IND<\/jats:styled-content>\n , and a negative correlation was determined between total\n I<\/jats:styled-content>\n gG and left ventricular ejection fraction (\n LVEF<\/jats:styled-content>\n ). Patients with\n IND<\/jats:styled-content>\n and\n CARD<\/jats:styled-content>\n presented similar higher levels of total\n I<\/jats:styled-content>\n gG specific to troponin\n T<\/jats:styled-content>\n and myosin than\n CONT<\/jats:styled-content>\n and\n ISCH<\/jats:styled-content>\n individuals. Patients with chronic\n C<\/jats:styled-content>\n hagas disease presented a negative correlation between left ventricular ejection fraction (\n LVEF<\/jats:styled-content>\n ) and the production of anti\u2010myosin and troponin\n T<\/jats:styled-content>\n autoantibodies. When grouped as low and high antibody producers and compared with\n LVEF<\/jats:styled-content>\n , we observed that high anti\u2010troponin T (\n P\u00a0<\/jats:italic>\n =\u00a00.042) and myosin (\n P<\/jats:italic>\n \u00a0=\u00a00.013) producers presented lower\n LVEF<\/jats:styled-content>\n than low producers. Moreover, there was a positive correlation (\n r<\/jats:italic>\n \u00a0=\u00a00.9508,\n P<\/jats:italic>\n \u00a0=\u00a00.0001) between the production of troponin\n T<\/jats:styled-content>\n and myosin autoantibodies.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n These findings indicate that increased production of anti\u2010cardiac troponin\n T<\/jats:styled-content>\n and myosin autoantibodies probably influences the left ventricular ejection fraction and could be related to chagasic cardiomyopathy.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/tmi.12169","type":"journal-article","created":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T07:25:41Z","timestamp":1375341941000},"page":"1180-1192","source":"Crossref","is-referenced-by-count":14,"title":["Troponin\n T<\/scp>\n autoantibodies correlate with chronic cardiomyopathy in human\n C<\/scp>\n hagas disease"],"prefix":"10.1111","volume":"18","author":[{"given":"Daniela Ferreira","family":"Nunes","sequence":"first","affiliation":[{"name":"Graduate Program in Health Sciences Center for Health Sciences Federal University of Rio Grande do Norte Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Marcos da Matta","family":"Guedes","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Parasitology Center for Biosciences Federal University of Rio Grande do Norte Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9ber","family":"de Mesquita Andrade","sequence":"additional","affiliation":[{"name":"Graduate Program in Health Sciences Center for Health Sciences Federal University of Rio Grande do Norte Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia Cl\u00e1udia J\u00e1come da","family":"C\u00e2mara","sequence":"additional","affiliation":[{"name":"Department of Clinical and Toxicological Analyses Center for Health Sciences Federal 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