{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:03:06Z","timestamp":1792803786077,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,9]],"date-time":"2014-01-09T00:00:00Z","timestamp":1389225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Johns Hopkins Center for Global Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>To evaluate the specificity of the Crystal <jats:styled-content style=\"fixed-case\">VC<\/jats:styled-content> dipstick test for detecting cholera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We compared direct testing using the Crystal <jats:styled-content style=\"fixed-case\">VC<\/jats:styled-content> dipstick test and testing after enrichment for 6 h in alkaline peptone water (<jats:styled-content style=\"fixed-case\">APW<\/jats:styled-content>) to bacterial culture as the gold standard. Samples positive by dipstick but negative by culture were also tested using <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Stool was collected from 125 patients. The overall specificities of the direct testing and testing after 6\u2010h enrichment in <jats:styled-content style=\"fixed-case\">APW<\/jats:styled-content> compared to bacterial culture were 91.8% and 98.4% (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.125), respectively, and the sensitivities were 65.6% and 75.0% (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.07), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The increase in the sensitivity of the Crystal <jats:styled-content style=\"fixed-case\">VC<\/jats:styled-content> kit with the use of the 6\u2010h enrichment step in <jats:styled-content style=\"fixed-case\">APW<\/jats:styled-content> compared to direct testing was marginally significant. The Crystal <jats:styled-content style=\"fixed-case\">VC<\/jats:styled-content> dipstick had a much higher specificity than previously reported (91\u201398%). Therefore, this method might be a promising screening tool for cholera outbreak surveillance in resource\u2010limited settings where elimination of false\u2010positive results is critical.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12252","type":"journal-article","created":{"date-parts":[[2014,1,9]],"date-time":"2014-01-09T06:16:49Z","timestamp":1389248209000},"page":"301-307","source":"Crossref","is-referenced-by-count":36,"title":["Evaluation of enrichment method for the detection of <i><scp>V<\/scp>ibrio cholerae <\/i><scp>O<\/scp>1 using a rapid dipstick test in <scp>B<\/scp>angladesh"],"prefix":"10.1111","volume":"19","author":[{"given":"Christine M.","family":"George","sequence":"first","affiliation":[{"name":"Department of International Health Program in Global Disease Epidemiology and Control Johns Hopkins Bloomberg School of Public Health Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahamud\u2010ur","family":"Rashid","sequence":"additional","affiliation":[{"name":"International Centre for Diarrhoeal Disease Research Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Sack","sequence":"additional","affiliation":[{"name":"Department of International Health Program in Global Disease Epidemiology and Control Johns Hopkins Bloomberg School of Public Health Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Bradley Sack","sequence":"additional","affiliation":[{"name":"Department of International Health Program in Global Disease Epidemiology and Control Johns Hopkins Bloomberg School of Public Health Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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