{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T13:37:42Z","timestamp":1793194662802,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T00:00:00Z","timestamp":1392163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Florida Department of Agriculture and Consumer Services Grant","award":["012018"],"award-info":[{"award-number":["012018"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health Grant","doi-asserted-by":"crossref","award":["AI-42164"],"award-info":[{"award-number":["AI-42164"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess vector competence (infection, dissemination and transmission) of <jats:italic>Culex pipiens quinquefasciatus<\/jats:italic> for Florida (FL) West Nile virus (WNV) isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>West Nile virus isolates (WN\u2010FL\u201003: NY99 genotype; WN\u2010FL\u201005\u2010558, WN\u2010FL\u201005\u20102186, WN\u2010FL\u201005\u2010510: WN02 genotype) collected from different regions of FL were used for vector competence experiments in <jats:italic>Cx.\u00a0p.\u00a0quinquefasciatus<\/jats:italic> from Alachua County and Indian River County in FL. Mosquitoes from both colonies were fed blood containing 7.9\u00a0\u00b1\u00a00.2 log<jats:sub>10<\/jats:sub> plaque\u2010forming units WNV\/ml\u00a0\u00b1\u00a0SE and incubated at 28\u00a0\u00b0C for 14\u00a0days. Vector competence, including rates of infection, dissemination, and transmission, was compared between colonies for WN\u2010FL\u201003 using <jats:italic>chi\u2010squared<\/jats:italic>. Virus titres in bodies, legs and saliva were compared using <jats:sc>anova<\/jats:sc>. Daily measurements of <jats:italic>in vitro<\/jats:italic> replication of WNV isolates were evaluated in Vero cells so that a standardised virus dose for each isolate could be delivered to mosquitoes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Infection and dissemination rates were high (\u226595%) and not affected by isolate or colony (infection, <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.679; dissemination, <jats:italic>P\u00a0=\u00a0<\/jats:italic>0.799). Transmission rates were low (\u226420%), detected in one colony and affected by isolate (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.008). Body and leg titres differed between isolates (body titre, <jats:italic>P\u00a0=\u00a0<\/jats:italic>0.031; leg titre, <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.044) and colonies (body titre, <jats:italic>P\u00a0=\u00a0<\/jats:italic>0.001; leg titre, <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.013) while saliva titre did not differ between isolates (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.462).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Variation in vector competence of mosquito populations may be attributed, in part, to exposures to WNV with genetic differences leading to different rates of replication in mosquitoes. Evaluation of vector competence for different WNV isolates may help us understand vector\u2013virus interactions and, hence, the role of vectors in complex virus transmission cycles in nature.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12277","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T12:34:59Z","timestamp":1392208499000},"page":"610-617","source":"Crossref","is-referenced-by-count":27,"title":["Vector competence of <i>Culex pipiens quinquefasciatus<\/i> (Diptera: Culicidae) for West Nile virus isolates from Florida"],"prefix":"10.1111","volume":"19","author":[{"given":"Stephanie L.","family":"Richards","sequence":"first","affiliation":[{"name":"Florida Medical Entomology Laboratory University of Florida Vero Beach FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheri L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Florida Medical Entomology Laboratory University of Florida Vero Beach FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia C.","family":"Lord","sequence":"additional","affiliation":[{"name":"Florida Medical Entomology Laboratory University of Florida Vero Beach FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2987\/09-5926.1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2987\/09-5935.1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.11-0473"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.032318-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2987\/8756-971X-28.4s.137"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002245"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000502"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres\/2009026"},{"key":"e_1_2_6_10_1","unstructured":"Centers for Disease Control and Prevention(2013)ArboNET Maps.http:\/\/www.cdc.gov\/ncidod\/dvbid\/westnile\/usgs_frame.html(accessed 28 May 2013)."},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-6-106"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.07.022"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1304.061473"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2004.71.493"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.04.029"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81015-0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1201010"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000092"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.11.4066-4071.2000"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.22.12717-12721.2004"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2012.10.023"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1705.101707"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.77.365"},{"issue":"75","key":"e_1_2_6_24_1","first-page":"42","article-title":"Monitoring and controlling West Nile virus: are your prevention practices in place?","volume":"13","author":"Nasci RS","year":"2013","journal-title":"Journal of Environmental Health"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2007.0101"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2009.81.264"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.09-0776"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02169-06"},{"key":"e_1_2_6_29_1","doi-asserted-by":"crossref","first-page":"951","DOI":"10.4269\/ajtmh.2008.79.951","article-title":"Short report: comparison of oral infectious dose of West Nile virus isolates representing three distinct genotypes in Culex quinquefasciatus","volume":"79","author":"Vanlandingham DL","year":"2008","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/45.3.483"}],"container-title":["Tropical Medicine &amp; 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