{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:52:43Z","timestamp":1789937563317,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,21]],"date-time":"2014-03-21T00:00:00Z","timestamp":1395360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Samsung Research Fund, Sungkyunkwan University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Neurocysticercosis (<jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>), an infection of the central nervous system with <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>aenia solium<\/jats:italic> metacestodes (<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>s<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>), invokes a formidable neurological disease. A bundle of antigens is applicable for serodiagnosis of active cases, while they demonstrate fairly low reactivity against sera of chronic <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>. Identification of sensitive biomarkers for chronic <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content> is critical for appropriate management of patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Proteome analysis revealed several isoforms of 65\u2010 and 83\u2010kDa TsM fasciclin\u2010like proteins (<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>s<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>as) to be highly reactive with sera of chronic <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>. A c<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> encoding one of the 83\u2010kDa Ts<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>as (Ts<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>as1) was isolated from a c<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> library. We expressed a recombinant protein (r<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>s<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>as1) and evaluated its diagnostic potential employing sera from chronic <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content> (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>80), tissue\u2010invasive cestodiases (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>169) and trematodiases (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>80) and those of normal controls (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>50).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Secretory Ts<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>as1 was composed of 766 amino acid polypeptide and harboured fasciclin and fasciclin\u2010superfamily domains. The protein was constitutively expressed in metacestode and adult stages, with preferential locality in the scolex. Bacterially expressed r<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>s<jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>as1 exhibited 78.8% sensitivity (63\/80 cases) and 93% specificity (278\/299 samples) in diagnosing chronic <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>. Some cross\u2010reactivity was observed with sera of cystic echinococcosis (10\/56, 17.8%) and sparganosis (4\/50, 8%). Positive and negative predictive values were 75% and 95.5%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TsM fasciclin\u2010like protein may be useful for differential diagnosis of chronic <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content> in clinical settings, especially where both <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content> and other infectious cerebral granulomatoses are prevalent.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12302","type":"journal-article","created":{"date-parts":[[2014,3,21]],"date-time":"2014-03-21T13:27:21Z","timestamp":1395408441000},"page":"719-725","source":"Crossref","is-referenced-by-count":10,"title":["<i>Taenia solium<\/i> metacestode fasciclin\u2010like protein is reactive with sera of chronic neurocysticercosis"],"prefix":"10.1111","volume":"19","author":[{"given":"Young\u2010An","family":"Bae","sequence":"first","affiliation":[{"name":"Department of Molecular Parasitology Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine and Center for Molecular Medicine Suwon Korea"},{"name":"Department of Microbiology Graduate School of Medicine Gachon University Incheon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joon\u2010Sup","family":"Yeom","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Kangbuk Samsung Hospital Sungkyunkwan University School of Medicine Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu","family":"Wang","sequence":"additional","affiliation":[{"name":"Qinghai Province Institute for Endemic Diseases Prevention and Control Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seon\u2010Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Molecular Parasitology Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine and Center for Molecular Medicine Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Seob","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Molecular Parasitology Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine and Center for Molecular Medicine Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Taek","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Molecular Parasitology Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine and Center for Molecular Medicine Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun\u2010Jong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Parasitology Ewha Womans University School of Medicine Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Molecular Parasitology Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine and Center for Molecular Medicine Suwon Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.200800084"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1586\/epr.10.72"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199808000-00007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(96)02640-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00454-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307235200"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-007-0507-x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(05)70194-0"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2008.10.014"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2011.07.004"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182005008504"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700332"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2011.10-0079"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1179\/136485906X78445"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001964"},{"key":"e_1_2_6_17_1","first-page":"399","article-title":"A proposal to declare neurocysticercosis an international reportable disease","volume":"78","author":"Roman G","year":"2000","journal-title":"Bulletin of World Health Organization"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1997.00440380101010"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.12.022"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1985.00360030074016"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199209033271005"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftmi.12302","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftmi.12302","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.12302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T21:36:00Z","timestamp":1696196160000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tmi.12302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,21]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/tmi.12302"],"URL":"https:\/\/doi.org\/10.1111\/tmi.12302","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,21]]}}}