{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T22:13:54Z","timestamp":1789856034025,"version":"4.0.1"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T00:00:00Z","timestamp":1397779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The immune trypanolysis test (<jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content>) is an accurate sero\u2010diagnostic tool increasingly implemented for sleeping sickness medical surveillance, but it is restricted to the reference laboratories. To facilitate storage and transport of the test specimen, we developed a protocol for the examination of blood spotted on filter paper (<jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content>\u2010fp) that can be stored and shipped at ambient temperature. We compared its performance with the classical <jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content> on plasma (<jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content>\u2010pl) that needs to be kept frozen until use.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study was conducted in active foci of the Republic of Guinea. In total, 438 specimens from treated and untreated sleeping sickness patients and serological suspects were tested with both methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content>\u2010fp gave significantly less positive results than <jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content>\u2010pl, but all the confirmed sleeping sickness cases were positive with the <jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content>\u2010fp protocol.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content>\u2010fp appears to offer a good compromise between feasibility and sensitivity to detect currently infected subjects who play a role in the transmission of <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>rypanosoma brucei gambiense<\/jats:italic> and is useful for contributing to the elimination of <jats:italic>gambiense<\/jats:italic> sleeping sickness.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12316","type":"journal-article","created":{"date-parts":[[2014,4,19]],"date-time":"2014-04-19T09:40:20Z","timestamp":1397900420000},"page":"828-831","source":"Crossref","is-referenced-by-count":24,"title":["Immune trypanolysis test with blood spotted on filter paper for epidemiological surveillance of sleeping sickness"],"prefix":"10.1111","volume":"19","author":[{"given":"Oumou","family":"Camara","sequence":"first","affiliation":[{"name":"Programme National de Lutte contre la Trypanosomose Humaine Africaine Conakry Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mamadou","family":"Camara","sequence":"additional","affiliation":[{"name":"Programme National de Lutte contre la Trypanosomose Humaine Africaine Conakry 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