{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:54:55Z","timestamp":1791251695097,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,5,19]],"date-time":"2015-05-19T00:00:00Z","timestamp":1431993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CP12\/03081"],"award-info":[{"award-number":["CP12\/03081"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI13\/01103"],"award-info":[{"award-number":["PI13\/01103"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess the prevalence and genetic diversity of the enteric protozoa species <jats:italic>G.\u00a0duodenalis<\/jats:italic>,<jats:italic> Cryptosporidium<\/jats:italic> spp. and <jats:italic>Entamoeba histolytica<\/jats:italic> in individuals with gastrointestinal symptoms compatible with infections by these pathogens seeking medical attention in a rural area in southern Ethiopia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 92 stool samples were initially screened by direct microscopy and immunochromatography and further confirmed by molecular methods. <jats:italic>G.\u00a0duodenalis<\/jats:italic>\u2010positive samples were molecularly characterised by multilocus genotyping of the glutamate dehydrogenase and <jats:italic>\u03b2<\/jats:italic>\u2010giardin genes of the parasite. <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequence analysis of the gene encoding the 60\u2010<jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a glycoprotein was used for the subtyping of <jats:italic>Cryptosporidium<\/jats:italic> isolates. Detection and differential diagnosis of <jats:italic>E.\u00a0histolytica<\/jats:italic>\/<jats:italic>dispar<\/jats:italic> were conducted by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010based prevalences were 10.9% for <jats:italic>G.\u00a0duodenalis<\/jats:italic>, 1.1% for <jats:italic>Cryptosporidium<\/jats:italic> spp. and 3.3% for <jats:italic>Entamoeba<\/jats:italic> spp. Seven (four novel and three known) subtypes of <jats:italic>G.\u00a0duodenalis<\/jats:italic> assemblage B were identified at the <jats:italic><jats:styled-content style=\"fixed-case\">GDH<\/jats:styled-content><\/jats:italic> locus and 5 (one novel and four known) at the <jats:italic><jats:styled-content style=\"fixed-case\">BG<\/jats:styled-content><\/jats:italic> locus. A novel variant of <jats:italic>C.\u00a0hominis<\/jats:italic> subtype IbA9G3 was also identified. Two <jats:italic>Entamoeba<\/jats:italic> isolates were assigned to <jats:italic>E.\u00a0dispar<\/jats:italic> and an additional one to <jats:italic>E.\u00a0histolytica<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although preliminary, our results strongly suggest that giardiasis, cryptosporidiosis and amoebiasis represent a significant burden in Ethiopian rural population.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12535","type":"journal-article","created":{"date-parts":[[2015,5,5]],"date-time":"2015-05-05T06:09:03Z","timestamp":1430806143000},"page":"1213-1222","source":"Crossref","is-referenced-by-count":32,"title":["Detection and molecular characterisation of <i>Giardia duodenalis<\/i>,<i> Cryptosporidium<\/i> spp. and <i>Entamoeba<\/i> spp. among patients with gastrointestinal symptoms in Gambo Hospital, Oromia Region, southern Ethiopia"],"prefix":"10.1111","volume":"20","author":[{"given":"Mar\u00eda J.","family":"Flecha","sequence":"first","affiliation":[{"name":"Hospital Pr\u00edncipe de Asturias Alcal\u00e1 de Henares Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia M.","family":"Benavides","sequence":"additional","affiliation":[{"name":"Hospital Pr\u00edncipe de Asturias Alcal\u00e1 de Henares Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Tissiano","sequence":"additional","affiliation":[{"name":"Gambo Rural General Hospital Shasehmane Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham","family":"Tesfamariam","sequence":"additional","affiliation":[{"name":"Gambo Rural General Hospital Shasehmane Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Cuadros","sequence":"additional","affiliation":[{"name":"Hospital Pr\u00edncipe de Asturias Alcal\u00e1 de Henares Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida","family":"de Lucio","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bego\u00f1a","family":"Bailo","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Cano","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Fuentes","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Carmena","sequence":"additional","affiliation":[{"name":"Centro Nacional de Microbiolog\u00eda Majadahonda Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,19]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"The World Health Report \u2013 1996, Fighting Disease Fostering Development","author":"WHO","year":"1996"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05279.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(00)01730-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07744-9"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874279301206010065"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2006.02.015"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2010.02.003"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-4-472"},{"key":"e_1_2_6_10_1","first-page":"392","article-title":"Different profile of intestinal protozoa and helminthic infections among patients with diarrhoea according to age attending a rural hospital in southern Ethiopia","volume":"31","author":"Ramos JM","year":"2014","journal-title":"Trop Biomed"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-13-151"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-14-166"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2008.02024.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002223"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-13-419"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072634"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/324329"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099559"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00033-10"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182014001085"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2007.04.003"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-8508(03)00057-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2004.02.001"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2004.10.022"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01067-06"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1360-2276.2003.01145.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1179\/136485910X12607012373759"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra022710"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis762"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2006.74.475"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-240"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.04.008"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001262"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2007.10.020"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2009.04.002"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12327"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2008.05.004"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftmi.12535","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.12535","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T10:10:49Z","timestamp":1696155049000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tmi.12535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,19]]},"references-count":37,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1111\/tmi.12535"],"URL":"https:\/\/doi.org\/10.1111\/tmi.12535","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,19]]}}}