{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T15:35:03Z","timestamp":1763480103225},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,3,17]],"date-time":"2016-03-17T00:00:00Z","timestamp":1458172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The introduction of leishmaniasis in a new area requires a well\u2010established population of the sandfly vector species of the parasite. No autochthonous cases of anthroponotic cutaneous leishmaniasis have been detected in southwestern Europe, and <jats:italic>Leishmania infantum<\/jats:italic> is the only causative agent of leishmaniasis in this area. <jats:italic>Phlebotomus sergenti,<\/jats:italic> the main vector of <jats:italic>Leishmania tropica,<\/jats:italic> is commonly found in the Iberian Peninsula at sufficient densities to be able to act as a vector. It is characterised by high genetic diversity and classified in four mitochondrial lineages. Our aim was to analyse the composition and distribution of <jats:italic>P. sergenti<\/jats:italic> mitochondrial lineages in southwestern Europe given the possibility of phenotypic differences of biomedical importance between them.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sandflies were captured in the Iberian Peninsula and on the Canary and Balearic Islands. Mitochondrial lineage identification of 137 <jats:italic>P. sergenti<\/jats:italic> was performed using a novel <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content> that avoids the necessity of gene sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two lineages were evidenced, the typical Iberian one (lineage I) and another, held in common with North Africa (lineage <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>), that show a distinctive distribution. <jats:italic>P. sergenti<\/jats:italic> lineage I shows a better correlation to the bioclimatic diversity in southwestern Europe. Conversely, <jats:italic>P. sergenti<\/jats:italic> lineage <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> prefers warmer temperatures and less precipitation, which are typical of the Mediterranean.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Lineage I seems to have adaptive advantages given its wider tolerance to temperature and altitude than lineage <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>, and it would seem more suitable to lead a potential geographical expansion towards the rest of Europe.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12686","type":"journal-article","created":{"date-parts":[[2016,2,27]],"date-time":"2016-02-27T13:10:35Z","timestamp":1456578635000},"page":"630-641","source":"Crossref","is-referenced-by-count":14,"title":["Differential ecological traits of two <i>Phlebotomus sergenti<\/i> mitochondrial lineages in southwestern Europe and their epidemiological 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