{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T02:36:49Z","timestamp":1787971009306,"version":"build-2784847793"},"reference-count":16,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,10,24]],"date-time":"2016-10-24T00:00:00Z","timestamp":1477267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the prevalence of asymptomatic cryptococcal antigen (<jats:styled-content style=\"fixed-case\">CRAG<\/jats:styled-content>) using lateral flow assay (<jats:styled-content style=\"fixed-case\">LFA<\/jats:styled-content>) in hospitalised <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4 counts &lt;200 cells\/<jats:italic>\u03bc<\/jats:italic>l.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Hospitalised <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients were prospectively recruited at Instituto de Infectologia Emilio Ribas, a tertiary referral hospital to <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients serving the S\u00e3o Paulo State, Brazil. All patients were &gt;18 years old without prior cryptococcal meningitis, without clinical suspicion of cryptococcal meningitis, regardless of antiretroviral (<jats:styled-content style=\"fixed-case\">ART<\/jats:styled-content>) status, and with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4 counts &lt;200 cells\/<jats:italic>\u03bc<\/jats:italic>l. Serum <jats:styled-content style=\"fixed-case\">CRAG<\/jats:styled-content> was tested by <jats:styled-content style=\"fixed-case\">LFA<\/jats:styled-content> in all patients, and whole blood <jats:styled-content style=\"fixed-case\">CRAG<\/jats:styled-content> was tested by <jats:styled-content style=\"fixed-case\">LFA<\/jats:styled-content> in positive cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We enrolled 163 participants of whom 61% were men. The duration of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> diagnosis was a median of 8 (range, 1\u201329) years. 26% were antiretroviral (<jats:styled-content style=\"fixed-case\">ART<\/jats:styled-content>)\u2010na\u00efve, and 74% were <jats:styled-content style=\"fixed-case\">ART<\/jats:styled-content>\u2010experienced. The median <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4 cell count was 25 (range, 1\u2013192) cells\/<jats:italic>\u03bc<\/jats:italic>l. Five patients (3.1%; 95%<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>, 1.0\u20137.0%) were asymptomatic <jats:styled-content style=\"fixed-case\">CRAG<\/jats:styled-content>\u2010positive. Positive results cases were cross\u2010verified by performing <jats:styled-content style=\"fixed-case\">LFA<\/jats:styled-content> in whole blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>3.1% of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected inpatients with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4 &lt;200 cells\/<jats:italic>\u03bc<\/jats:italic>l without symptomatic meningitis had cryptococcal antigenemia in S\u00e3o Paulo, suggesting that routine <jats:styled-content style=\"fixed-case\">CRAG<\/jats:styled-content> screening may be beneficial in similar settings in South America. Our study reveals another targeted population for <jats:styled-content style=\"fixed-case\">CRAG<\/jats:styled-content> screening: hospitalised <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4 &lt;200 cells\/<jats:italic>\u03bc<\/jats:italic>l, regardless of <jats:styled-content style=\"fixed-case\">ART<\/jats:styled-content> status. Whole blood <jats:styled-content style=\"fixed-case\">CRAG LFA<\/jats:styled-content> screening seems to be a simple strategy to prevention of symptomatic meningitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12790","type":"journal-article","created":{"date-parts":[[2016,10,11]],"date-time":"2016-10-11T16:14:27Z","timestamp":1476202467000},"page":"1539-1544","source":"Crossref","is-referenced-by-count":37,"title":["Asymptomatic cryptococcal antigen prevalence detected by lateral flow assay in hospitalised HIV\u2010infected patients in S\u00e3o Paulo, Brazil"],"prefix":"10.1111","volume":"21","author":[{"given":"Jos\u00e9 E.","family":"Vidal","sequence":"first","affiliation":[{"name":"Instituto de Infectologia Emilio Ribas S\u00e3o Paulo Brazil"},{"name":"Faculdade de Medicina Hospital das Cl\u00ednicas da Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Instituto de Medicina Tropical da Universidade de S\u00e3o Paulo S\u00e3o Paulo 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