{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T16:08:41Z","timestamp":1748448521564},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,2]],"date-time":"2016-11-02T00:00:00Z","timestamp":1478044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006301","name":"Center for International Business Education and Research, University of Illinois at Urbana-Champaign","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006301","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To present and evaluate simple, cost\u2010effective tests to determine the amount of insecticide on treated materials.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We developed and evaluated a competitive immunoassay on two different platforms: a label\u2010free impedimetric biosensor (<jats:styled-content style=\"fixed-case\">EIS<\/jats:styled-content> biosensor) and a lateral flow. Both approaches were validated by gas chromatography (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, gold standards for analytical methods and immunoassays, respectively. Finally, commercially available pyrethroid\u2010treated <jats:styled-content style=\"fixed-case\">ITN<\/jats:styled-content> samples were analysed. Different extraction methods were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Insecticide extraction by direct infusion of the <jats:styled-content style=\"fixed-case\">ITN<\/jats:styled-content> samples with dichloromethane and dioxane showed recovery efficiencies around 100% for insecticide\u2010coated bednets, and &gt;70% for insecticide\u2010incorporated bednets. These results were comparable to those obtained with standard sonication methods. The competitive immunoassay characterisation with <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> presented a dynamic range between 12 n<jats:sc>m<\/jats:sc> and 1.5 \u03bc<jats:sc>m<\/jats:sc> (coefficient of variation (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>) below 5%), with an <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub> at 138 n<jats:sc>m<\/jats:sc>, and a limit of detection (<jats:styled-content style=\"fixed-case\">LOD<\/jats:styled-content>) of 3.2 n<jats:sc>m<\/jats:sc>. <jats:styled-content style=\"fixed-case\">EIS<\/jats:styled-content> biosensor had a linear range between 1.7 n<jats:sc>m<\/jats:sc> and 61 n<jats:sc>m<\/jats:sc> (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> around 14%), with an <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub> at 10.4 n<jats:sc>m<\/jats:sc>, and a <jats:styled-content style=\"fixed-case\">LOD<\/jats:styled-content> of 0.6 n<jats:sc>m<\/jats:sc>. Finally, the lateral flow approach showed a dynamic range between 150 n<jats:sc>m<\/jats:sc> and 1.5 \u03bc<jats:sc>m<\/jats:sc>, an <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub> at 505 n<jats:sc>m<\/jats:sc> and a LOD of 67 n<jats:sc>m<\/jats:sc>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> can replace chromatography as an accurate laboratory technique to determine insecticide concentration in bednets. The lateral flow approach developed can be used to estimate <jats:styled-content style=\"fixed-case\">ITN<\/jats:styled-content> insecticide concentration in the field. This new technology, coupled to the new extraction methods, should provide reliable guidelines for <jats:styled-content style=\"fixed-case\">ITN<\/jats:styled-content> use and replacement in the field.<\/jats:p><\/jats:sec>","DOI":"10.1111\/tmi.12794","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T05:51:30Z","timestamp":1476337890000},"page":"41-51","source":"Crossref","is-referenced-by-count":5,"title":["Assessment of analytical methods to determine pyrethroids content of bednets"],"prefix":"10.1111","volume":"22","author":[{"given":"Marc","family":"Castellarnau","sequence":"first","affiliation":[{"name":"ISGlobal Barcelona Centre for International Health Research (CRESIB) Hospital Cl\u00ednic \u2013 Universitat de Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Ram\u00f3n\u2010Azc\u00f3n","sequence":"additional","affiliation":[{"name":"Nanobiotechnology for Diagnostics Institute for Advanced Chemistry of Catalonia of the Spanish Council for Scientific Research Barcelona Spain"},{"name":"CIBER de Bioingenier\u00eda Biomateriales y Nanomedicina Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yolanda","family":"Gonzalez\u2010Quinteiro","sequence":"additional","affiliation":[{"name":"Institute of Environmental Assessment and Water Research Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi F.","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Institute of Environmental Assessment and Water Research Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan O.","family":"Grimalt","sequence":"additional","affiliation":[{"name":"Institute of Environmental Assessment and Water Research Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda\u2010Pilar","family":"Marco","sequence":"additional","affiliation":[{"name":"Nanobiotechnology for Diagnostics Institute for Advanced Chemistry of Catalonia of the Spanish Council for Scientific Research Barcelona Spain"},{"name":"CIBER de Bioingenier\u00eda Biomateriales y Nanomedicina Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Nieuwenhuijsen","sequence":"additional","affiliation":[{"name":"ISGlobal Centre for Research in Environmental Epidemiology (CREAL) Universitat Pompeu Fabra (UPF) CIBER Epidemiolog\u00eda y Salud P\u00fablica (CIBERESP) Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Picado","sequence":"additional","affiliation":[{"name":"ISGlobal Barcelona Centre for International Health Research (CRESIB) Hospital Cl\u00ednic \u2013 Universitat de Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003228"},{"key":"e_1_2_9_3_1","unstructured":"Safety of Pyrethroids for Public Health Use.WHO Pesticide Evaluation Scheme 2005."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-9-84"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/272051"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2011.10.014"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.04.004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114915"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-011-0601-6"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2007.04.009"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2009.05.008"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/03067310903359526"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2012.03.042"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/s130810167"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004324"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-178"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2009.02247.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2011.05.040"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2011.04.024"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0207629"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx034220c"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5184-z"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2014.03.020"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2012.01.036"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4109(200103)13:3<173::AID-ELAN173>3.0.CO;2-B"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2004.12.020"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac060533b"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.06.057"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2007.12.010"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.08.032"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-3661-4"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2009.03.020"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/03067318408077000"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/B712609C"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4005(03)00096-0"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng086"},{"key":"e_1_2_9_37_1","unstructured":"International Agency for Research on Cancer: IARC Monographs on the Evaluation of Carcinogenic Risks to Humans 2016.http:\/\/monographs.iarc.fr\/ENG\/Classification\/latest_classif.php."},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-240-3_2"},{"key":"e_1_2_9_39_1","unstructured":"World Health Organization: Guidelines for the Laboratory and Field Testing of Long\u2010Lasting Insecticidal Mosquito Nets 2005.http:\/\/apps.who.int\/iris\/bitstream\/10665\/69007\/1\/WHO_CDS_WHOPES_GCDPP_2005.11.pdf."},{"key":"e_1_2_9_40_1","unstructured":"Innovative Vector Control Consortium (IVCC) 2016.http:\/\/www.ivcc.com\/creating-solutions."}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftmi.12794","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftmi.12794","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tmi.12794","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T02:29:35Z","timestamp":1696213775000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tmi.12794"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,2]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1111\/tmi.12794"],"URL":"https:\/\/doi.org\/10.1111\/tmi.12794","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,2]]}}}